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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Vimentin Antibody/SPC-1338D-RPE/100-µl
商品详细StressMarq/Anti-Vimentin Antibody/SPC-1338D-RPE/100-µl
StressMarq/Anti-Vimentin Antibody/SPC-1338D-RPE/100-µl
StressMarq/Anti-Vimentin Antibody/SPC-1338D-RPE/100-µl
商品编号: SPC-1338D-RPE
市场价: ¥5980.00
美元价: 3588.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameVimentinAntibody
Description

RabbitAnti-HumanVimentinPolyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ELISA(1:10000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromInternalofhumanvimentin.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~53kDa,endogenouslevelsoftotalvimentinprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1338,RRID:AB_2713871
CertificateofAnalysisA1:1000dilutionofSPC-1338wassufficientfordetectionofVimentinin10µgofHepG2celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesCTRCT30Antibody,Epididymisluminalprotein113Antibody,HEL113Antibody,VIMAntibody,VIME_HUMANAntibody,VimentinAntibody
ResearchAreasCancer,CellMarkers,CellSignaling,CellStructure,Neurodegeneration,Neuroscience
CellularLocalizationCytoplasm
AccessionNumberNP_003371.2
GeneID7431
SwissProtP08670
ScientificBackgroundVimentinisatypeIIIintermediatefilament(IF)protein.AllIFproteinsarehighlyexpressedandvimentinisthemajorcytoskeletalcomponentofmesechymal,andthereforeVimentinisusedasamarkerofmesenchymally-derivedcellsorcellsundergoingEMTduringbothnormaldevelopmentandmetasticprogression.Functionallyitalsoservesasignificantroleinsupportingandanchordingtheorganellesinthecytosol.Clinicalsignificanceincludesbeingusedasasarcomatumormarker,andithasalsobeenusedinthediagnosisofrheumatoidarthritis.
References1.KatsumotoT.,MitsushimaA.,KurimuraT.(1990).BiolCell.68(2):139–146.
2.LeaderM,CollinsM,PatelJ,HenryK(1987).Histopathology11(1):63–72.
3."ImmunohistochemistryfromtheWashingtonAnimalDiseaseDiagnosticlaboratory(WADDL)oftheCollegeofVeterinaryMedicine,WashingtonStateUniversity".Retrieved2009-03-14.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-VimentinPolyclonalAntibody(SPC-1338).Tissue:ColonCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-VimentinPolyclonalAntibody(SPC-1338)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa Vimentin protein using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Lane 1: Human HepG2 cells, treated with Adriamycin (0.5uM, 5hours). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:1000. Predicted/Observed Size: ~54kDa.</p>

WesternblotanalysisofHumanHepG2celllysatesshowingdetectionof~54kDaVimentinproteinusingRabbitAnti-VimentinPolyclonalAntibody(SPC-1338).Lane1:HumanHepG2cells,treatedwithAdriamycin(0.5uM,5hours).Lane2:Untreated.PrimaryAntibody:RabbitAnti-VimentinPolyclonalAntibody(SPC-1338)at1:1000.Predicted/ObservedSize:~54kDa.

ProductCitations(0)

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 R-PE(R-Phycoerythrin)
Overview:
  • Broadexcitationspectrum
  • Highquantumyield
  • Photostable
  • Memberofthephycobiliproteinfamily
  • Isolatedfromredalgae
  • Excellentsolubilityinwater
  • MolecularWeight:250kDa

R-PEDatasheet

 R-PE Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =565nm

λem=575nm

εmax=2.0×106

Φf= 0.84

Brightness=1.68x103

Laser = 488to561nm

Filterset = TRITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。