StressMarq/Anti-Vimentin Antibody/SPC-1338D-BI/100-µl-免疫在线蚂蚁淘旗下平台

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StressMarq/Anti-Vimentin Antibody/SPC-1338D-BI/100-µl

商品编号： SPC-1338D-BI

品牌： StressMarq Biosciences

市场价： ¥5960.00

美元价： 3576.00

产地：美国(厂家直采)

公司：

产品分类：电泳缓冲液

公司分类： Electrophoretic_buffer

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

Product Name Vimentin Antibody

Description

Rabbit Anti-Human Vimentin Polyclonal

Species Reactivity Human, Mouse, Rat

Applications WB, IHC, ELISA

Antibody Dilution WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthesized peptide derived from Internal of human vimentin.

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects ~53kDa, endogenous levels of total vimentin protein.
Cite This Product	StressMarq Biosciences Cat# SPC-1338, RRID: AB_2713871
Certificate of Analysis	A 1:1000 dilution of SPC-1338 was sufficient for detection of Vimentin in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
Research Areas	Cancer, Cell Markers, Cell Signaling, Cell Structure, Neurodegeneration, Neuroscience
Cellular Localization	Cytoplasm
Accession Number	NP_003371.2
Gene ID	7431
Swiss Prot	P08670
Scientific Background	Vimentin is a type III intermediate filament (IF) protein. All IF proteins are highly expressed and vimentin is the major cytoskeletal component of mesechymal, and therefore Vimentin is used as a marker of mesenchymally-derived cells or cells undergoing EMT during both normal development and metastic progression. Functionally it also serves a significant role in supporting and anchording the organelles in the cytosol. Clinical significance includes being used as a sarcoma tumor marker, and it has also been used in the diagnosis of rheumatoid arthritis.
References	1. Katsumoto T., Mitsushima A., Kurimura T. (1990). Biol Cell. 68(2): 139–146. 2. Leader M, Collins M, Patel J, Henry K (1987). Histopathology 11(1): 63–72. 3. "Immunohistochemistry from the Washington Animal Disease Diagnostic laboratory (WADDL)of the College of Veterinary Medicine, Washington State University". Retrieved 2009-03-14.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa Vimentin protein using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Lane 1: Human HepG2 cells, treated with Adriamycin (0.5uM, 5hours). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:1000. Predicted/Observed Size: ~54kDa.</p>

Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa Vimentin protein using Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338). Lane 1: Human HepG2 cells, treated with Adriamycin (0.5uM, 5hours). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Vimentin Polyclonal Antibody (SPC-1338) at 1:1000. Predicted/Observed Size: ~54kDa.

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Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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