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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl
商品详细StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl
StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl
StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl
商品编号: SMC-261D-P594
市场价: ¥6180.00
美元价: 3708.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameVimentinAntibody
Description

MouseAnti-HumanVimentinMonoclonalIgG1

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,ICC/IF
AntibodyDilutionWB(1:2000),ICC/IF(1:200);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesMouse
ImmunogenSpeciesHuman
ImmunogenFull-lengthprotein.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBS,pH7.4,50%glyercol,0.05%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityMonoclonal
CloneNumber1A7
IsotypeIgG1
SpecificityAntibodycandetectsendogenousVimentinprotein.
CiteThisProductStressMarqBiosciencesCat#SMC-261,RRID:AB_2699844
CertificateofAnalysisA1:2000dilutionofSMC-261wassufficientfordetectionofVimentinin10µgofHuman,Mouse,Rat,HeLa,BraincelllysatesbyECLimmunoblotanalysisusingGoatAnti-MouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesCTRCT30Antibody,Epididymisluminalprotein113Antibody,HEL113Antibody,VIMAntibody,VIME_HUMANAntibody,VimentinAntibody
CellularLocalizationCytoplasm
AccessionNumberNP_003371.2
GeneID7431
SwissProtP08670
ScientificBackgroundVimentinisatypeIIIintermediatefilament(IF)protein.AllIFproteinsarehighlyexpressedandvimentinisthemajorcytoskeletalcomponentofmesechymal,andthereforeVimentinisusedasaMarkerofmesenchymally-derivedcellsorcellsundergoingEMTduringbothnormaldevelopmentandmetasticprogression.Functionallyitalsoservesasignificantroleinsupportingandanchordingtheorganellesinthecytosol.Clinicalsignificanceincludesbeingusedasasarcomatumormarker,andithasalsobeenusedinthediagnosisofrheumatoidarthritis.
References1.KatsumotoT.,MitsushimaA.,KurimuraT.(1990).BiolCell.68(2):139–146.
2.LeaderM,CollinsM,PatelJ,HenryK(1987).Histopathology11(1):63–72.
3.ImmunohistochemistryfromtheWashingtonAnimalDiseaseDiagnosticlaboratory(WADDL)oftheCollegeofVeterinaryMedicine,WashingtonStateUniversity.Retrieved2009-03-14.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Tissue: HeLa cell lysates. Species: Human. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-VimentinMonoclonalAntibody,Clone1A7(SMC-261).Tissue:HeLacelllysates.Species:Human.PrimaryAntibody:MouseAnti-VimentinMonoclonalAntibody(SMC-261)at1:100.SecondaryAntibody:FITCGoatAnti-Mouse(green).Counterstain:DAPI(blue)nuclearstain.

<p>Western Blot analysis of Human, Mouse, Rat HeLa, Brain cell lysates showing detection of ~50-57 kDa Vimentin protein using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Lane 1: Human HeLa. Lane 2: Mouse Brain. Lane 3: Rat Brain.. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:2000. Predicted/Observed Size: ~50-57 kDa.</p>

WesternBlotanalysisofHuman,Mouse,RatHeLa,Braincelllysatesshowingdetectionof~50-57kDaVimentinproteinusingMouseAnti-VimentinMonoclonalAntibody,Clone1A7(SMC-261).Lane1:HumanHeLa.Lane2:MouseBrain.Lane3:RatBrain..PrimaryAntibody:MouseAnti-VimentinMonoclonalAntibody(SMC-261)at1:2000.Predicted/ObservedSize:~50-57kDa.

ProductCitations(0)

Currentlytherearenocitationsforthisproduct.

 PE/ATTO594
PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.
Overview:
  • Highfluorescenceyield
  • HighphotostABIlity
  • Veryhydrophilic
  • Excellentsolubilityinwater
  • Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission SpectraOpticalProperties:

λex =535nm

λem =627 nm

Laser = 488to561nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。