StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl

StressMarq/抗波形蛋白抗体[1A7]/SMC-261D-P594/100-µl

商品编号： SMC-261D-P594

品牌： StressMarq Biosciences

市场价： ¥6180.00

美元价： 3708.00

产地：美国(厂家直采)

公司：

产品分类：电泳缓冲液

公司分类： Electrophoretic_buffer

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Overview:

ProductName VimentinAntibody

Description

MouseAnti-HumanVimentinMonoclonalIgG1

SpeciesReactivity Human,Mouse,Rat

Applications WB,ICC/IF

AntibodyDilution WB(1:2000),ICC/IF(1:200);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Mouse

ImmunogenSpecies Human

Immunogen Full-lengthprotein.

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBS,pH7.4,50%glyercol,0.05%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Clonality	Monoclonal
CloneNumber	1A7
Isotype	IgG1
Specificity	AntibodycandetectsendogenousVimentinprotein.
CiteThisProduct	StressMarqBiosciencesCat#SMC-261,RRID:AB_2699844
CertificateofAnalysis	A1:2000dilutionofSMC-261wassufficientfordetectionofVimentinin10µgofHuman,Mouse,Rat,HeLa,BraincelllysatesbyECLimmunoblotanalysisusingGoatAnti-MouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	CTRCT30Antibody,Epididymisluminalprotein113Antibody,HEL113Antibody,VIMAntibody,VIME_HUMANAntibody,VimentinAntibody
CellularLocalization	Cytoplasm
AccessionNumber	NP_003371.2
GeneID	7431
SwissProt	P08670
ScientificBackground	VimentinisatypeIIIintermediatefilament(IF)protein.AllIFproteinsarehighlyexpressedandvimentinisthemajorcytoskeletalcomponentofmesechymal,andthereforeVimentinisusedasaMarkerofmesenchymally-derivedcellsorcellsundergoingEMTduringbothnormaldevelopmentandmetasticprogression.Functionallyitalsoservesasignificantroleinsupportingandanchordingtheorganellesinthecytosol.Clinicalsignificanceincludesbeingusedasasarcomatumormarker,andithasalsobeenusedinthediagnosisofrheumatoidarthritis.
References	1.KatsumotoT.,MitsushimaA.,KurimuraT.(1990).BiolCell.68(2):139–146. 2.LeaderM,CollinsM,PatelJ,HenryK(1987).Histopathology11(1):63–72. 3.ImmunohistochemistryfromtheWashingtonAnimalDiseaseDiagnosticlaboratory(WADDL)oftheCollegeofVeterinaryMedicine,WashingtonStateUniversity.Retrieved2009-03-14.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Tissue: HeLa cell lysates. Species: Human. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-VimentinMonoclonalAntibody,Clone1A7(SMC-261).Tissue:HeLacelllysates.Species:Human.PrimaryAntibody:MouseAnti-VimentinMonoclonalAntibody(SMC-261)at1:100.SecondaryAntibody:FITCGoatAnti-Mouse(green).Counterstain:DAPI(blue)nuclearstain.

<p>Western Blot analysis of Human, Mouse, Rat HeLa, Brain cell lysates showing detection of ~50-57 kDa Vimentin protein using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Lane 1: Human HeLa. Lane 2: Mouse Brain. Lane 3: Rat Brain.. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:2000. Predicted/Observed Size: ~50-57 kDa.</p>

WesternBlotanalysisofHuman,Mouse,RatHeLa,Braincelllysatesshowingdetectionof~50-57kDaVimentinproteinusingMouseAnti-VimentinMonoclonalAntibody,Clone1A7(SMC-261).Lane1:HumanHeLa.Lane2:MouseBrain.Lane3:RatBrain..PrimaryAntibody:MouseAnti-VimentinMonoclonalAntibody(SMC-261)at1:2000.Predicted/ObservedSize:~50-57kDa.

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PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

公司介绍