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当前位置： 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Vimentin Antibody [1A7]/SMC-261D-HRP/100-µl
商品详细StressMarq/Anti-Vimentin Antibody [1A7]/SMC-261D-HRP/100-µl
StressMarq/Anti-Vimentin Antibody [1A7]/SMC-261D-HRP/100-µl
StressMarq/Anti-Vimentin Antibody [1A7]/SMC-261D-HRP/100-µl
商品编号： SMC-261D-HRP
市场价： ¥5800.00
美元价： 3480.00
产地： 美国(厂家直采)
公司：
产品分类： 电泳缓冲液
公司分类： Electrophoretic_buffer
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name Vimentin Antibody
Description

Mouse Anti-Human Vimentin Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, ICC/IF
Antibody Dilution WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Full-length protein.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Monoclonal
Clone Number 1A7
Isotype IgG1
Specificity Antibody can detects endogenous Vimentin protein.
Cite This Product StressMarq Biosciences Cat# SMC-261, RRID: AB_2699844
Certificate of Analysis A 1:2000 dilution of SMC-261 was sufficient for detection of Vimentin in 10 µg of Human, Mouse, Rat, HeLa, Brain cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
Cellular Localization Cytoplasm
Accession Number NP_003371.2
Gene ID 7431
Swiss Prot P08670
Scientific Background Vimentin is a type III intermediate filament (IF) protein. All IF proteins are highly expressed and vimentin is the major cytoskeletal component of mesechymal, and therefore Vimentin is used as a marker of mesenchymally-derived cells or cells undergoing EMT during both normal development and metastic progression. Functionally it also serves a significant role in supporting and anchording the organelles in the cytosol. Clinical significance includes being used as a sarcoma tumor marker, and it has also been used in the diagnosis of rheumatoid arthritis.
References 1. Katsumoto T., Mitsushima A., Kurimura T. (1990). Biol Cell. 68(2): 139–146.
2. Leader M, Collins M, Patel J, Henry K (1987). Histopathology 11(1): 63–72.
3. Immunohistochemistry from the Washington Animal Disease Diagnostic laboratory (WADDL)of the College of Veterinary Medicine, Washington State University. Retrieved 2009-03-14.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Tissue: HeLa cell lysates. Species: Human. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Tissue: HeLa cell lysates. Species: Human. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.

<p>Western Blot analysis of Human, Mouse, Rat HeLa, Brain cell lysates showing detection of ~50-57 kDa Vimentin protein using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Lane 1: Human HeLa. Lane 2: Mouse Brain. Lane 3: Rat Brain.. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:2000. Predicted/Observed Size: ~50-57 kDa.</p>

Western Blot analysis of Human, Mouse, Rat HeLa, Brain cell lysates showing detection of ~50-57 kDa Vimentin protein using Mouse Anti-Vimentin Monoclonal Antibody, Clone 1A7 (SMC-261). Lane 1: Human HeLa. Lane 2: Mouse Brain. Lane 3: Rat Brain.. Primary Antibody: Mouse Anti-Vimentin Monoclonal Antibody (SMC-261) at 1:2000. Predicted/Observed Size: ~50-57 kDa.

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HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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