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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-VGLUT2 Antibody [S29-29]/SMC-395D-STR/100-µg
商品详细StressMarq/Anti-VGLUT2 Antibody [S29-29]/SMC-395D-STR/100-µg
StressMarq/Anti-VGLUT2 Antibody [S29-29]/SMC-395D-STR/100-µg
StressMarq/Anti-VGLUT2 Antibody [S29-29]/SMC-395D-STR/100-µg
商品编号: SMC-395D-STR
市场价: ¥6780.00
美元价: 4068.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name VGLUT2 Antibody
Description

Mouse Anti-Rat VGLUT2 Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S29-29
Isotype IgG1
Specificity Detects ~60kDa.
Cite This Product StressMarq Biosciences Cat# SMC-395, RRID: AB_11229706
Certificate of Analysis 1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Differentiation associated BNPI Antibody, DNPI Antibody, SLC17A6 Antibody, Solute carrier family 17 member 6 Antibody, Vesicular glutamate transporter 2 Antibody, Differentiation associated Na dependent inorganic phosphate cotransporter Antibody, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 Antibody, Differentiation associated Na(+) dependent inorganic phosphate cotransporter Antibody, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter Antibody, Sodium dependent inorganic phosphate cotransporter Antibody
Research Areas Cell Markers, Cell Signaling, Cell Structure, Neuron Markers, Neuroscience, Neurotransmitter Transporters, Pre-Synaptic Markers, Presynaptic Markers, Pumps/Transporters
Cellular Localization Cell Junction, Cytoplasmic Vesicle, Membrane, Secretory vesicle, Synapse, Synaptic vesicle membrane
Accession Number NP_445879.1
Gene ID 84487
Swiss Prot Q9JI12
Scientific Background The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
References 1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625.
2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250.
3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451.
4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803.
5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391.
6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.

Product Images

<p>Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:000.</p>

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:000.

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.

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Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。