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StressMarq/Anti-VGLUT2 Antibody [S29-29]/SMC-395D-RPE/100-µg
Overview:
Product Name | VGLUT2 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Rat VGLUT2 Monoclonal IgG1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | S29-29 |
Isotype | IgG1 |
Specificity | Detects ~60kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-395, RRID: AB_11229706 |
Certificate of Analysis | 1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | Differentiation associated BNPI Antibody, DNPI Antibody, SLC17A6 Antibody, Solute carrier family 17 member 6 Antibody, Vesicular glutamate transporter 2 Antibody, Differentiation associated Na dependent inorganic phosphate cotransporter Antibody, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 Antibody, Differentiation associated Na(+) dependent inorganic phosphate cotransporter Antibody, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter Antibody, Sodium dependent inorganic phosphate cotransporter Antibody |
Research Areas | Cell Markers, Cell Signaling, Cell Structure, Neuron Markers, Neuroscience, Neurotransmitter Transporters, Pre-Synaptic Markers, Presynaptic Markers, Pumps/Transporters |
Cellular Localization | Cell Junction, Cytoplasmic Vesicle, Membrane, Secretory vesicle, Synapse, Synaptic vesicle membrane |
Accession Number | NP_445879.1 |
Gene ID | 84487 |
Swiss Prot | Q9JI12 |
Scientific Background | The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI. |
References |
1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814. |
Product Images
Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:000.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.
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R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |