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StressMarq/Anti-VE Cadherin Antibody/SPC-1290D-A488/100-µl
Overview:
Product Name | VE Cadherin Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human VE Cadherin Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:2000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | A synthesized peptide derived from human VE-Cadherin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Affinity Purified |
Clonality | Polyclonal |
Isotype | IgG |
Specificity | Detects endogenous levels of total VE-Cadherin |
Cite This Product | StressMarq Biosciences Cat# SPC-1290, RRID: AB_2713025 |
Certificate of Analysis | A 1:1000 dilution of SPC-1290 was sufficient for detection of VE Cadherin in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | 7B4 Antibody, Cadherin 5 Antibody, Cadherin 5 type 2 Antibody, cadherin, vascular endothelial Antibody, CD 144 Antibody, CDH 5 Antibody, Endothelial specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE Cad Antibody |
Research Areas | Cancer, Cardiovascular Markers, Cardiovascular System, Cell adhesion, Cell Signaling, Cytoskeleton, Endothelial Cells, Invasion/microenvironment |
Cellular Localization | Cell Junction, Cell membrane |
Accession Number | NP_001786.2 |
Gene ID | 1003 |
Swiss Prot | P33151 |
Scientific Background | Cadherins are a super family of transmembrane glycoproteins that contain cadherin repeats of ~ 100 residues in their extracellular domain. They mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. Specifically, VE Cadherin plays an inportant role in endothelial cell biology. It associates with alpha-catenin forming a link to the cytoskeleton. And its signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. |
References |
1. Wheelock M.J. and Johnson K.R. (2003) Annu Rev Cell Dev Biol. 19: 207-235. 2. Rabascio C., et al. (2004) Cancer Res. 64: 4373-4377. 3. Yamaoka-Tojo M., et al. (2006) Arterioscler Thromb Vasc Biol. 26: 1991-1997. |
Product Images
Western blot analysis of Human Jurkat cell lysates showing detection of ~120kDa VE Cadherin protein using Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290) at 1:1000. Predicted/Observed Size: ~120kDa.
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ATTO 488 | ||
Overview:
ATTO 488 Datasheet | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |