StressMarq/StressXpress®泛素相互作用检测试剂盒/SKT-132-20/20分析
商品编号：
SKT-132-20
市场价：
¥7900.00
美元价：
4740.00
产品分类：
电泳缓冲液
公司分类：
Electrophoretic_buffer
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | UbiquitinInteractDetectionKit |
| Description | Captureanddetectionofubiquitinbindingproteins |
| SpeciesReactivity | SpeciesIndependent |
| Platform | SpinColumn |
| SampleTypes | Celllysates,Tissue |
| AssayType | BindingMatrix/QuantitativeWB(WesternBlot) |
| Utility | Detectionkitusedtocapture,detect,identifyandcharacteriseisolatedubiquitinbindingproteinsbyWesternblottingorproteomicanalysisinsamples. |
| IncubationTime | 1hour |
| NumberofSamples | 20samples |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||
| ProductType | DetectionKits | ||||||||||||
| AssayOverview | TheUbiquitinInteractDetectionkitfacilitatestheselectivecaptureanddetectionofubiquitinbindingandassociatedproteinsfromcelllysatesandtissueextracts.Thekitutilisesahighcapacity,highspecificityubiquitinmatrixtogetherwithaneasy-to-usepurificationsystemforefficientisolationofubiquitinbindingproteinswithminimalnon-specificbinding.Allowspurificationofmono-andpoly-ubiquitinbindingproteins,independentofchainlinkageorlength.AnalysisbyWesternblottingorproteomicmethodsenablesidentificationandassessmentofcapturedproteins.Kitcontainssufficientubiquitininteractmatrixtoperformupto20bindingassays. | ||||||||||||
| KitOverview |
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| CiteThisProduct | UbiquitinInteractDetectionKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-132) |
BIOLOGicalDescription
| AlternativeNames | PolyubiquitinBDetectionKit,RPS27ADetectionKit,UBA52DetectionKit,UBBDetectionKit,UBCDetectionKit,ubiquitinBDetectionKit |
| ResearchAreas | Cancer,Apoptosis,Autophagy,CardiovascularSystem,CellSignaling,Epigenetics,Neurodegeneration,Neuroscience,Post-translationalModifications,Ubiquitination |
| ScientificBackground | Thecovalentattachmentofubiquitintoproteins(ubiquitination)playsafundamentalroleintheregulationofcellularfunctionthroughbiologicaleventsincludingcellcycle,differentiation,immuneresponses,DNArepair,chromatinstructure,transcription,signaltransduction,endocytosis,apoptosisanddegradationbytheproteasome,autophagyandlysosomesystems.Assuchubiquitinsignallingandtheprocessesitmediatesareessentialforthenormalfunctioningofcellsanditsdysfunctionhasbeenimplicatedinwiderangeofdiseasesincludingcancer,neurodegeneration,cardiovascularandmetabolicdisorders(1,2).Thetypeofubiquitinmodification,(monoubiquitin,multiubiquitin,polyubiquitin),substrateproteinlysineresidue(s)modifiedand,inthecaseofpolyubiquitination,thechainlengthandlysinelinkagetypecontrolthefunctionandfateofubiquitinatedproteins.Inadditionallubiquitinmediatedpathwaysalsoutilisespecificubiquitinreceptorstofacilitatetheirregulation(3,4).Ubiquitinationisachievedthroughthreeenzymaticsteps.InanATP-dependentprocess,theubiquitinE1activatingenzymecatalysestheformationofareactivethioesterbondwithubiquitin,followedbyitssubsequenttransfertotheactivesitecysteineofaubiquitinE2carrierprotein.TheselectivityoftheubiquitincascadeforaparticularsubstrateproteinreliesontheinteractionbetweentheE2conjugatingenzyme(ofwhichacellcontainsrelativelyfew)andanubiquitinE3ligase,ofwhichover600havebeenidentifiedtodate.ThespecificE2-E3pairrequiredforubiquitinationofaparticularsubstrateproteininvivomayalsocontrolthetype,pointandlength/linkage(polyubiquitin)oftheubiquitinmodification(5). |
| References | 1.Fulda,S.,Rajalingam,K.&Dikic,I.EMBOmolecularmedicine4,545–56(2012). 2.Shaid,S.,Brandts,C.H.,Serve,H.&Dikic,I.Celldeathanddifferentiation20,21–30(2013). 3.Husnjak,K.&Dikic,I.Annualreviewofbiochemistry81,291–322(2012). 4.Komander,D.&Rape,M.AnnualReviewofBiochemistry81,203–229(2012). 5.Spasser,L.&Brik,A.AngewandteChemie(Internationaled.inEnglish)51,6840–62(2012). |
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
