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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Ubiquitin Antibody [Ubi-1]/SMC-215D-A390/100-µl
商品详细StressMarq/Anti-Ubiquitin Antibody [Ubi-1]/SMC-215D-A390/100-µl
StressMarq/Anti-Ubiquitin Antibody [Ubi-1]/SMC-215D-A390/100-µl
StressMarq/Anti-Ubiquitin Antibody [Ubi-1]/SMC-215D-A390/100-µl
商品编号: SMC-215D-A390
市场价: ¥7460.00
美元价: 4476.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Ubiquitin Antibody
Description

Mouse Anti-Bovine Ubiquitin Monoclonal IgG1

Species Reactivity Species Independent
Applications WB, IHC, ICC/IF, ELISA
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Bovine
Immunogen Bovine ubiquitin, KLH-conjugate
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer Ascitic fluid containing 10mM sodium azide
Storage Temperature -20ºC, 4ºC
Shipping Temperature Blue Ice or 4ºC
Purification Ascites
Clonality Monoclonal
Clone Number Ubi-1
Isotype IgG1
Specificity Detects free ubiquitin, polyubiquitin chains and ubiquitinated proteins.
Cite This Product StressMarq Biosciences Cat# SMC-215, RRID: AB_2570422
Certificate of Analysis A 1:1000 dilution of SMC-215 was sufficient for detection of ubiquitin conjugates and free ubiquitin in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Research Areas Cell Signaling, Post-translational Modifications, Ubiquitination
Cellular Localization Cytoplasm, Nucleus
Accession Number BAC56955.1
Gene ID 7314
Swiss Prot P0CG47
Scientific Background Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
References 1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12.
2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone Ubi-1 (SMC-215). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-215) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone Ubi-1 (SMC-215). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-215) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.

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  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOptical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。