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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A633/100-µl
商品详细StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A633/100-µl
StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A633/100-µl
StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A633/100-µl
商品编号: SPC-1337D-A633
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameTyrosineHydroxylaseAntibody
Description

RabbitAnti-HumanTyrosineHydroxylasePolyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ICC/IF,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:500),ELISA(1:10000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedunphosphorylatedpeptidederivedfromhumanTyrosineHydroxylasearoundthephosphorylationsiteofserine19(A-V-SP-E-Q).
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~60kDa,endogenouslevelsoftotalTyrosineHydroxylaseprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1337,RRID:AB_2713853
CertificateofAnalysisA1:1000dilutionofSPC-1337wassufficientfordetectionofTyrosineHydroxylasein10µgof3T3celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesDystonia14Antibody,DYT14Antibody,DYT5bAntibody,ProteinPaleAntibody,TYHAntibody,Tyrosine3hydroxylaseAntibody,Tyrosine3monooxygenaseAntibody,TyrosinehydroxylaseAntibody
ResearchAreasCancer,Neuroscience,NeurotransmitterReceptors
AccessionNumberNP_000351.2
GeneID7054
SwissProtP07101
ScientificBackgroundTyrosinehydroxylase,alsoknownastyrosine3-monooxygenase,istheenzymethatconvertsaminoacidL-TyrosinetoL-3,4-Dihydroxyphenylalanine(L-DOPA).L-DOPAisaprecursortodopamine,whichisthenconvertedtonorepinephrineandepinephrine(adrenaline).Dopamine,norepinephrineandepinephrineareallimportantneurotransmitters.AlterationsintheactivityoftyrosinehydroxylasemaybeinvolvedinanumberofneurologicaldiseasessuchasParkinson"sandschizophrenia.Thisenzymeisalsorequiredforembryonicdevelopment.
References1.KumerS.C.andVranaK.E.(1996)JNeuRochem.67:443-462.
2.Bodeau-PéanS.,etal.(1999)JBiolChem.274:3469-3475.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-TyrosineHydroxylasePolyclonalAntibody(SPC-1337).Tissue:BrainTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-TyrosineHydroxylasePolyclonalAntibody(SPC-1337)at1:1000.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Mouse 3T3 cell lysates showing detection of ~60kDa Tyrosine Hydroxylase protein using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Lane 1: Mouse 3T3 cells treated with Forskolin (40nM, 30min). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. Predicted/Observed Size: ~60kDa.</p>

WesternblotanalysisofMouse3T3celllysatesshowingdetectionof~60kDaTyrosineHydroxylaseproteinusingRabbitAnti-TyrosineHydroxylasePolyclonalAntibody(SPC-1337).Lane1:Mouse3T3cellstreatedwithForskolin(40nM,30min).Lane2:Untreated.PrimaryAntibody:RabbitAnti-TyrosineHydroxylasePolyclonalAntibody(SPC-1337)at1:1000.Predicted/ObservedSize:~60kDa.

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 ATTO633
Overview:
  • Highfluorescenceyield
  • HighthermalandphotostABIlity
  • Moderatelyhydrophilic
  • Goodsolubilityinpolarsolvents
  • StableatpH4–11
  • Cationicdye,perchloratesalt
  • MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission SpectrumOpticalProperties:

λex =629nm

λem=657nm

εmax=1.3×105

Φf= 0.64

τfl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。