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当前位置： 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A488/100-µl
商品详细StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A488/100-µl
StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A488/100-µl
StressMarq/Anti-Tyrosine Hydroxylase Antibody/SPC-1337D-A488/100-µl
商品编号： SPC-1337D-A488
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 电泳缓冲液
公司分类： Electrophoretic_buffer
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name Tyrosine Hydroxylase Antibody
Description

Rabbit Anti-Human Tyrosine Hydroxylase Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized unphosphorylated peptide derived from human Tyrosine Hydroxylase around the phosphorylation site of serine 19 (A-V-SP-E-Q).
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~60kDa, endogenous levels of total Tyrosine Hydroxylase protein.
Cite This Product StressMarq Biosciences Cat# SPC-1337, RRID: AB_2713853
Certificate of Analysis A 1:1000 dilution of SPC-1337 was sufficient for detection of Tyrosine Hydroxylase in 10 µg of 3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Dystonia 14 Antibody, DYT14 Antibody, DYT5b Antibody, Protein Pale Antibody, TYH Antibody, Tyrosine 3 hydroxylase Antibody, Tyrosine 3 monooxygenase Antibody, Tyrosine hydroxylase Antibody
Research Areas Cancer, Neuroscience, Neurotransmitter Receptors
Accession Number NP_000351.2
Gene ID 7054
Swiss Prot P07101
Scientific Background Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline). Dopamine, norepinephrine and epinephrine are all important neurotransmitters. Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia. This enzyme is also required for embryonic development.
References 1. Kumer S.C. and Vrana K.E. (1996) J Neurochem. 67: 443-462.
2. Bodeau-Péan S., et al. (1999) J Biol Chem. 274: 3469-3475.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Mouse 3T3 cell lysates showing detection of ~60kDa Tyrosine Hydroxylase protein using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Lane 1: Mouse 3T3 cells treated with Forskolin (40nM, 30min). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. Predicted/Observed Size: ~60kDa.</p>

Western blot analysis of Mouse 3T3 cell lysates showing detection of ~60kDa Tyrosine Hydroxylase protein using Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337). Lane 1: Mouse 3T3 cells treated with Forskolin (40nM, 30min). Lane 2: Untreated. Primary Antibody: Rabbit Anti-Tyrosine Hydroxylase Polyclonal Antibody (SPC-1337) at 1:1000. Predicted/Observed Size: ~60kDa.

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  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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