StressMarq/Anti-TNF-R1 Antibody/SPC-170D-FITC/100-µg-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-TNF-R1 Antibody/SPC-170D-FITC/100-µg

StressMarq/Anti-TNF-R1 Antibody/SPC-170D-FITC/100-µg

商品编号： SPC-170D-FITC

品牌： StressMarq Biosciences

市场价： ¥6320.00

美元价： 3792.00

产地：美国(厂家直采)

公司：

产品分类：电泳缓冲液

公司分类： Electrophoretic_buffer

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName TNF-R1Antibody

Description

RabbitAnti-MouseTNF-R1Polyclonal

SpeciesReactivity Dog,Human,Monkey,Mouse,Rat,Bovine,Rabbit

Applications WB,IHC,ICC/IF,IP

AntibodyDilution WB(1:1000),IHC(1:100),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Mouse

Immunogen PeptidecorrespondingtoAA20-43ofthemouseTNF-R1sequence,identicaltoratandhumanoverthoseresidues

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects~55kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-170,RRID:AB_2703803
CertificateofAnalysis	1µg/mlofSPC-170wassufficientfordetectionofTNFR1in20µgofHelalysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	Tumornecrosisfactorreceptor1Antibody,TNFR-1Antibody,TNFRSF1AAntibody,TNFARAntibody,TNFR1Antibody
ResearchAreas	Cancer,Apoptosis,Atherosclerosis,CardiovascularSystem,CellSignaling
CellularLocalization	Cellmembrane,Golgiapparatus,GolgiApparatusMembrane
AccessionNumber	P19438
GeneID	9606
SwissProt	P19438
ScientificBackground	TheTumorNecrosisFactorReceptor(TNFR)alsoknownasClusterofdifferentiation(CD120)isaproteinthatbelongstothe(TNF)/(TNFR)superfamily.TNFinteractswithtwodistinctreceptorsTNFR1andTNFR2.Thesereceptorssharenohomologyontheircytoplasmicsequences(1,3).TNFR1alsoknownasp55/p60isahighaffinityreceptorforTNF-α.TheTNFR1hasanextracellulardomainwithvariablenumbersofcysteine-richrepeats.ThefunctionalpropertiesofTNFR1aretargetsinnewtherapiesforosteoporosis,chronicinflammatoryandautoimmunediseases(1,2).TheTNF-α/TNFR1receptorcomplexisresponsIBLefortherecruitmentandthesubsequentactivationofthecaspase(aspartate-specificcysteineproteases)thatregulateapoptosis.
References	1.KontermannR.E.,etal.(2008)JImmunother.31(3):225-34. 2.HehlgansT.andPfefferK.(2005)Immunology.115(1):1-20. 3.Al-LamkiS.,etal.(2005)TheFasebJournal.19:1638-1645.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-TNF-R1PolyclonalAntibody(SPC-170).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-TNF-R1PolyclonalAntibody(SPC-170)at1:100for12hoursat4°C.SecondaryAntibody:APCGoatAnti-Rabbit(red)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Golgiapparatusmembrane.Magnification:100x.(A)DAPI(blue)nuclearstain.(B)Anti-TNF-R1Antibody.(C)Composite.

<p>Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis.</p>

ImmunohistochemistryanalysisusingRabbitAnti-TNF-R1PolyclonalAntibody(SPC-170).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeSolution.PrimaryAntibody:RabbitAnti-TNF-R1PolyclonalAntibody(SPC-170)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:50for1houratRT.Localization:dermis.

<p>Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.</p>

WesternblotanalysisofMouseLivercelllysatesshowingdetectionof~55kDaTNF-R1proteinusingRabbitAnti-TNF-R1PolyclonalAntibody(SPC-170).Lane1:MolecularWeightLadder(MW).Lane2:MouseLivercelllysates.Load:15µg.Block:5%SkimMilkin1XTBST.PrimaryAntibody:RabbitAnti-TNF-R1PolyclonalAntibody(SPC-170)at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-RabbitIgG:HRPat1:2000for60minatRT.ColorDevelopment:ECLsolutionfor5minatRT.Predicted/ObservedSize:~55kDa.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-TNF-R1PolyclonalAntibody(SPC-170).Tissue:HaCaTcells.Species:Human.Fixation:Cold100%methanolat-20Cfor10minutes.PrimaryAntibody:RabbitAnti-TNF-R1PolyclonalAntibody(SPC-170)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbitat1:50for1-2hoursatRTindark.Localization:Punctatenuclearstaining,dottystainingincytoplasm.

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-TNF-R1PolyclonalAntibody(SPC-170).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-TNF-R1PolyclonalAntibody(SPC-170)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Golgiapparatusmembrane.Magnification:20x.(A)DAPI(blue)nuclearstain.(B)Anti-TNF-R1Antibody.(C)Composite.

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FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

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