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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-TNF alpha Antibody/SPC-1315D-HRP/100-µl
商品详细StressMarq/Anti-TNF alpha Antibody/SPC-1315D-HRP/100-µl
StressMarq/Anti-TNF alpha Antibody/SPC-1315D-HRP/100-µl
StressMarq/Anti-TNF alpha Antibody/SPC-1315D-HRP/100-µl
商品编号: SPC-1315D-HRP
市场价: ¥5800.00
美元价: 3480.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameTNFalphaAntibody
Description

RabbitAnti-HumanTNFalphaPolyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsIHC,ICC/IF,ELISA
AntibodyDilutionIHC(1:100),ICC/IF(1:500),ELISA(1:10000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfrominternalofhumanTNFA.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsoftotalTNFAprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1315,RRID:AB_2713457
CertificateofAnalysisA1:100dilutionofSPC-1315wassufficientfordetectionofTNFalphainhumanHepG2cellsusingGoatAnti-RabbitIgGasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesAPC1Antibody,APC1proteinAntibody,CachectinAntibody,DIFAntibody,DifferentiationinducingfactorAntibody,MacrophagecytotoxicfactorAntibody,TNFalphaAntibody,TnfAntibody,TNFsuperfamily,member2Antibody,TNF,macrophagederivedAntibody,TNF,monocytederivedAntibody,TNF-aAntibody,TNF-alphaAntibody,TNFAAntibody,TNFA_HUMANAntibody,TNFSF2Antibody,TumornecrosisfactoralphaAntibody,TumornecrosisfactorAntibody,Tumornecrosisfactorligandsuperfamilymember2Antibody,TumorNecrosisFactor,MembraneFormAntibody,Tumornecrosisfactor,solubleformAntibody
CellularLocalizationCellmembrane
AccessionNumberNP_000585.2
GeneID7124
SwissProtP01375
ScientificBackgroundTumornecrosisfactorisacellsignalingprotein(cytokine)involvedinsystemicinflammationandisoneofthecytokinesthatmakeuptheacutephasereaction.TheprimaryroleofTNFisintheregulationofimmunecells.TNF,beinganendogenouspyrogen,isabletoinducefever,apoptoticcelldeath,cachexia,inflammationandtoinhibittumOrigenesisandviralreplicationandrespondtosepsisviaIL1&IL6producingcells.DysregulationofTNFproductionhasbeenimplicatedinavarietyofhumandiseasesincludingAlzheimer"sdisease,cancer,majordepression,Psoriasisandinflammatoryboweldisease(IBD).
References1.SwardfagerWetal.(2010)BiolPsychiatry68(10):930–941.
2.LocksleyRMetal.(2001)Cell104(4):487–501.
3.DowlatiYetal.(2010)BiolPsychiatry67(5):446–457.
4.VictorFCandGottliebAB(2002)JDrugsDermatol1(3):264–75.
5.BrynskovJ.(2002)Gut51(1):37–43.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-TNFalphaPolyclonalAntibody(SPC-1315).Tissue:ColonCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-TNFalphaPolyclonalAntibody(SPC-1315)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-TNFalphaPolyclonalAntibody(SPC-1315).Tissue:HepG2cells.Species:Human.PrimaryAntibody:RabbitAnti-TNFalphaPolyclonalAntibody(SPC-1315)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

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HRP(HorserADIshperoxidase)

Properties:

  • Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
  • Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
  • Catalyzestheconversionof:
    • Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
    • Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
    • Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
  • Highturnoverrateenablesrapidgenerationofastrongsignal
  • 44kDaglycoprotein
  • Extinctioncoefficient:100(403nm)
  • Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。