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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-FITC/100-µl
商品详细StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-FITC/100-µl
StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-FITC/100-µl
StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-FITC/100-µl
商品编号: SPC-1312D-FITC
市场价: ¥5880.00
美元价: 3528.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameTGFbeta1Antibody
Description

RabbitAnti-HumanTGFbeta1Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ELISA(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumanTGFβ1antibody.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~45kDa,endogenouslevelsoftotalTGFβ1protein.
CiteThisProductStressMarqBiosciencesCat#SPC-1312,RRID:AB_2713403
CertificateofAnalysisA1:1000dilutionofSPC-1312wassufficientfordetectionofTGFbeta1in10µgofHepG2celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesCEDAntibody,DPD1Antibody,LAPAntibody,Latency-associatedpeptideAntibody,Preprotransforminggrowthfactorbeta1Antibody,TGFbeta1Antibody,TGFbetaAntibody,TGFbeta1proteinAntibody,TGF-beta1proteinAntibody,TGF-beta-1Antibody,TGF-beta-5Antibody,TGF-beta1Antibody,TGFBAntibody,Tgfb-1Antibody,tgfb1Antibody,TGFB1_HUMANAntibody,TGFbetaAntibody,TGFbeta1Antibody,TransformingGrowthFactorb1Antibody,TransformingGrowthFactorbeta1Antibody,Transforminggrowthfactorbeta1aAntibody,transforminggrowthfactorbeta-1Antibody,transforminggrowthfactor,beta1Antibody
CellularLocalizationSecreted
AccessionNumberNP_000651.3
GeneID7040
SwissProtP01137
ScientificBackgroundTransforminggrowthfactorbeta1orTGF-β1isapolypeptidememberofthetransforminggrowthfactorbetasuperfamilyofcytokines.ManycellshaveTGFBreceptors,andtheproteinpositivelyandnegativelyregulatesmanyothergrowthfactors.Thesecretedproteiniscleavedintoalatency-associatedpeptide(LAP)andamatureTGFB1peptide,andisfoundineitheralatentformcomposedofaTGFB1homodimer,aLAPhomodimer,andalatentTGFB1-bindingprotein,orinanactiveformcomposedofaTGFB1homodimer.ThematurepeptidemayalsoformheterodimerswithotherTGFBfamilymembers.Thisgeneisfrequentlyupregulatedintumorcells,andmutationsinthisgeneresultinCamurati-Engelmanndisease.Itplaysanimportantroleinboneremodelingasitisapotentstimulatorofosteoblasticboneformation,causingchemotaxis,proliferationanddifferentiationincommittedosteoblasts.
References1.GhadamiM.etal.(2000)Am.J.Hum.Genet.66(1):143–7.
2.VaughnSP.etal.(2000)Genomics66(1):119–21.
3.AssoianRK.etal.(1983)J.Biol.Chem.258(11):7155–60.
4.WahlSMetal.(1988)J.Immunol.140(9):3026–32.
5.TiemessenMMetal.(2003)Int.Immunol.15(12):1495–504.
6.McGonigleSetal.(2001)FEBSLett.490(1-2):65–9.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-TGFbeta1PolyclonalAntibody(SPC-1312).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-TGFbeta1PolyclonalAntibody(SPC-1312)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HepG2 cell lysates showing detection of ~44kDa TGF beta 1 protein using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:1000. Predicted/Observed Size: ~44kDa.</p>

WesternblotanalysisofHumanHepG2celllysatesshowingdetectionof~44kDaTGFbeta1proteinusingRabbitAnti-TGFbeta1PolyclonalAntibody(SPC-1312).Lane1:HumanHepG2cells.Lane2:HumanHepG2cellstreatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-TGFbeta1PolyclonalAntibody(SPC-1312)at1:1000.Predicted/ObservedSize:~44kDa.

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 FITC(Fluorescein)
Overview:
  • Excellentfluorescencequantumyield
  • Highrateofphotobleaching
  • Goodsolubilityinwater
  • Broademissionspectrum
  • pHdependentspectra
  • Molecularformula:C20H12O5
  • Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =494 nm

λem=520 nm

εmax=7.3×104

Φf= 0.92

τfl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。