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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-A700/100-µl
商品详细StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-A700/100-µl
StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-A700/100-µl
StressMarq/Anti-TGF beta 1 Antibody/SPC-1312D-A700/100-µl
商品编号： SPC-1312D-A700
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name TGF beta 1 Antibody
Description

Rabbit Anti-Human TGF beta 1 Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human TGF β1 antibody.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~45kDa, endogenous levels of total TGF β1 protein.
Cite This Product StressMarq Biosciences Cat# SPC-1312, RRID: AB_2713403
Certificate of Analysis A 1:1000 dilution of SPC-1312 was sufficient for detection of TGF beta 1 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names CED Antibody, DPD1 Antibody, LAP Antibody, Latency-associated peptide Antibody, Prepro transforming growth factor beta 1 Antibody, TGF beta 1 Antibody, TGF beta Antibody, TGF beta 1 protein Antibody, TGF-beta 1 protein Antibody, TGF-beta-1 Antibody, TGF-beta-5 Antibody, TGF-beta1 Antibody, TGFB Antibody, Tgfb-1 Antibody, tgfb1 Antibody, TGFB1_HUMAN Antibody, TGFbeta Antibody, TGFbeta1 Antibody, Transforming Growth Factor b1 Antibody, Transforming Growth Factor beta 1 Antibody, Transforming growth factor beta 1a Antibody, transforming growth factor beta-1 Antibody, transforming growth factor, beta 1 Antibody
Cellular Localization Secreted
Accession Number NP_000651.3
Gene ID 7040
Swiss Prot P01137
Scientific Background Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
References 1. Ghadami M. et al. (2000) Am. J. Hum. Genet. 66 (1): 143–7.
2. Vaughn SP. et al. (2000) Genomics 66 (1): 119–21.
3. Assoian RK. et al. (1983) J. Biol. Chem. 258 (11): 7155–60.
4. Wahl SM et al. (1988) J. Immunol. 140 (9): 3026–32.
5. Tiemessen MM et al. (2003) Int. Immunol. 15 (12): 1495–504.
6. McGonigle S et al. (2001) FEBS Lett. 490 (1-2): 65–9.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human HepG2 cell lysates showing detection of ~44kDa TGF beta 1 protein using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:1000. Predicted/Observed Size: ~44kDa.</p>

Western blot analysis of Human HepG2 cell lysates showing detection of ~44kDa TGF beta 1 protein using Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-TGF beta 1 Polyclonal Antibody (SPC-1312) at 1:1000. Predicted/Observed Size: ~44kDa.

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 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

 ATTO 700 Fluorophore Absorption and Emission SpectrumOptical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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