|
Antibody Dilution |
WB (1:1000); optimal dilutions for assays should be determined by the user. |
|
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
| PerCP | Overview:- Peridinin-Chlorophyll-Protein Complex
- Small phycobiliprotein
- Isolated from red algae
- Large stokes shift (195 nm)
- Molecular Weight: 35 kDa
PerCP Datasheet |  | Optical Properties: λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| PE/ATTO 594 | | PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | Overview:- High fluorescence yield
- High photostability
- Very hydrophilic
- Excellent solubility in water
- Very little aggregation
PE/ATTO 594 Datasheet |  | Optical Properties: λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| FITC (Fluorescein) | Overview:- Excellent fluorescence quantum yield
- High rate of photobleaching
- Good solubility in water
- Broad emission spectrum
- pH dependent spectra
- Molecular formula: C20H12O5
- Molar mass: 332.3 g/mol
FITC-Fluorescent-conjugate |  | Optical Properties: λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| ATTO 700 | Overview:- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
ATTO 700 Datasheet |  | Optical Properties: λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| ATTO 680 | Overview:- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 631 g/mol
ATTO 680 Datasheet |  | Optical Properties: λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 655 | Overview:- High fluorescence yield
- High thermal and photostability
- Excellent ozone resistance
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 634 g/mol
ATTO 655 Datasheet |  | Optical Properties: λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 633 | Overview:- High fluorescence yield
- High thermal and photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Stable at pH 4 – 11
- Cationic dye, perchlorate salt
- Molar Mass: 652.2 g/mol
ATTO 633 Datasheet |  | Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 594 | Overview:- High fluorescence yield
- High photostability
- Very hydrophilic
- Excellent solubility in water
- Very little aggregation
- New dye with net charge of -1
- Molar Mass: 1137 g/mol
ATTO 594 Datasheet |  | Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 565 | Overview:- High fluorescence yield
- High thermal and photostability
- Good solubility in polar solvents
- Excellent solubility in water
- Very little aggregation
- Rhodamine dye derivative
- Molar Mass: 611 g/mol
ATTO 565 Datasheet |  | Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 488 | Overview:- High fluorescence yield
- High photostability
- Very hydrophilic
- Excellent solubility in water
- Very little aggregation
- New dye with net charge of -1
- Molar Mass: 804 g/mol
ATTO 488 Datasheet |  | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 390 | Overview:- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet |  | Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| APC (Allophycocyanin) | Overview:- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
APC Datasheet |  | Optical Properties: λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
StreptavidinProperties: - Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Streptavidin Datasheet Biotin Properties: - Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
- Molar mass: 244.31 g/mol
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin Datasheet HRP (Horseradish peroxidase)Properties: - Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
HRP Datasheet AP (Alkaline Phosphatase)Properties: - Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
AP Datasheet | R-PE (R-Phycoerythrin) | Overview:- Broad excitation spectrum
- High quantum yield
- Photostable
- Member of the phycobiliprotein family
- Isolated from red algae
- Excellent solubility in water
- Molecular Weight: 250 kDa
R-PE Datasheet |  | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
|
