AntibodyDilution WB(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser. Conjugates AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview: Peridinin-Chlorophyll-ProteinComplex Smallphycobiliprotein Isolatedfromredalgae Largestokesshift(195nm) MolecularWeight:35kDa PerCPDatasheet
OpticalProperties: λex =482nm
λem =677nm
εmax =1.96x106
Laser =488nm
PE/ATTO594 PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. Overview: Highfluorescenceyield HighphotostABI Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation PE/ATTO594Datasheet
OpticalProperties: λex =535nm
λem =627 nm
Laser = 488to561nm
FITC(Fluorescein) Overview: Excellentfluorescencequantumyield Highrateofphotobleaching Goodsolubilityinwater Broademissionspectrum pHdependentspectra Molecularformula:C20 H12 O5 Molarmass:332.3g/mol FITC-Fluorescent-conjugate
OpticalProperties: λex =494 nm
λem =520 nm
εmax =7.3×104
Φf = 0.92
τfl =5.0ns
Brightness = 67.2
Laser = 488 nm
Filterset = FITC
ATTO700 Overview: Highfluorescenceyield Excellentthermalandphotostability Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:575g/mol ATTO700Datasheet
OpticalProperties: λex =700 nm
λem =719nm
εmax =1.25×105
Φf = 0.25
τfl =1.6ns
Brightness = 31.3
Laser =676nm
Filterset =Cy®5.5
ATTO680 Overview: Highfluorescenceyield Excellentthermalandphotostability Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:631g/mol ATTO680Datasheet
OpticalProperties: λex =680nm
λem =700 nm
εmax =1.25×105
Φf = 0.30
τfl =1.7ns
Brightness =37.5
Laser =633 –676nm
Filterset =Cy®5.5
ATTO655 Overview: Highfluorescenceyield Highthermalandphotostability Excellentozoneresistance Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:634g/mol ATTO655Datasheet
OpticalProperties: λex =663nm
λem =684nm
εmax =1.25×105
Φf = 0.30
τfl =1.8ns
Brightness = 37.5
Laser =633 –647nm
Filterset =Cy®5
ATTO633 Overview: Highfluorescenceyield Highthermalandphotostability Moderatelyhydrophilic Goodsolubilityinpolarsolvents StableatpH4–11 Cationicdye,perchloratesalt MolarMass:652.2g/mol ATTO633Datasheet
OpticalProperties: λex =629nm
λem =657nm
εmax =1.3×105
Φf = 0.64
τfl =3.2ns
Brightness =83.2
Laser =633 nm
Filterset =Cy®5
ATTO594 Overview: Highfluorescenceyield Highphotostability Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation Newdyewithnetchargeof-1 MolarMass:1137g/mol ATTO594Datasheet
OpticalProperties: λex =601 nm
λem =627nm
εmax =1.2×105
Φf = 0.85
τfl =3.5ns
Brightness =102
Laser =594nm
Filterset =TexasRed®
ATTO565 Overview: Highfluorescenceyield Highthermalandphotostability Goodsolubilityinpolarsolvents Excellentsolubilityinwater Verylittleaggregation Rhodaminedyederivative MolarMass:611g/mol ATTO565Datasheet
OpticalProperties: λex =563nm
λem =592nm
εmax =1.2×105
Φf = 0.9
τfl =3.4n
Brightness =10
Laser =532nm
Filterset = TRITC
ATTO488 Overview: Highfluorescenceyield Highphotostability Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation Newdyewithnetchargeof-1 MolarMass:804g/mol ATTO488Datasheet
OpticalProperties: λex =501nm
λem =523nm
εmax =9.0×104
Φf = 0.80
τfl =4.1ns
Brightness = 72
Laser = 488 nm
Filterset = FITC
ATTO390 Overview: Highfluorescenceyield LargeStokes-shift(89nm) Goodphotostability Moderatelyhydrophilic Goodsolubilityinpolarsolvents Coumarinderivate,uncharged Lowmolarmass:343.42g/mol ATTO390Datasheet
OpticalProperties: λex =390nm
λem =479nm
εmax =2.4×104
Φf = 0.90
τfl =5.0ns
Brightness = 21.6
Laser =365or405nm
APC (Allophycocyanin)Overview: Highquantumyield Largephycobiliprotein 6chromophorespermolecule Isolatedfromredalgae MolecularWeight:105kDa APCDatasheet
OpticalProperties: λex =650nm
λem =660nm
εmax =7.0×105
Φf = 0.68
Brightness = 476
Laser =594or633 nm
Filterset =Cy®5
Streptavidin Properties:
Homo-tetramericproteinpurifiedfromStreptomycesavidinii whichbindsfourbiotinmoleculeswithextremelyhighaffinity Molecularweight:53kDa Formula:C10 H16 N2 O3 S Applications:Westernblot,immunohistochemistry,andELISA StreptavidinDatasheet
Biotin Properties:
BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity Molarmass:244.31g/mol Formula:C10 H16 N2 O3 S Applications:Westernblot,immunohistochemistry,andELISA BiotinDatasheet
HRP(HorserADI Properties:
Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget Readilycombineswithhydrogenperoxide(H2 O2 )toformHRP-H2 O2 complexwhichcanoxidizevarioushydrogendonors Catalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL) Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts Highturnoverrateenablesrapidgenerationofastrongsignal 44kDaglycoprotein Extinctioncoefficient:100(403nm) Applications:Westernblot,immunohistochemistry,andELISA HRPDatasheet
AP(AlkalinePhosphatase) Properties:
Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation Catalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts Molecularweight:140kDa Applications:Westernblot,immunohistochemistry,andELISA APDatasheet
R-PE (R-Phycoerythrin)Overview: Broadexcitationspectrum Highquantumyield Photostable Memberofthephycobiliproteinfamily Isolatedfromredalgae Excellentsolubilityinwater MolecularWeight:250kDa R-PEDatasheet
OpticalProperties: λex =565nm
λem =575nm
εmax =2.0×106
Φf = 0.84
Brightness =1.68x103
Laser = 488to561nm
Filterset = TRITC
ProductImages Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-TASK1PotassiumChannelMonoclonalAntibody,CloneS374-48(SMC-473).Tissue:NeuroblastomacelllineSK-N-BE.Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:MouseAnti-TASK1PotassiumChannelMonoclonalAntibody(SMC-473)at1:100for60minatRT.SecondaryAntibody:GoatAnti-MouseATTO488at1:100for60minatRT.Counterstain:PhalloidinTexasRedF-Actinstain;DAPI(blue)nuclearstainat1:1000,1:5000for60minRT,5minRT.Localization:Membrane.Magnification:60X.(A)DAPI(blue)nuclearstain(B)PhalloidinTexasRedF-Actinstain(C)TASK1PotassiumChannelAntibody(D)Composite.
WesternBlotanalysisofRatBrainMembraneshowingdetectionof~50kDaTASK1PotassiumChannelproteinusingMouseAnti-TASK1PotassiumChannelMonoclonalAntibody,CloneS374-48(SMC-473).Lane1:MolecularWeightLadder(MW).Lane2:Ratbrainmembrane.Load:15µg.Block:2%BSAand2%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-TASK1PotassiumChannelMonoclonalAntibody(SMC-473)at1:1000for16hoursat4°C.SecondaryAntibody:GoatAnti-MouseIgG:HRPat1:2000for60minatRT.ColorDevelopment:ECLsolutionfor6minatRT.Predicted/ObservedSize:~50kDa.
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ATTO565 Overview: Highfluorescenceyield Highthermalandphotostability Goodsolubilityinpolarsolvents Excellentsolubilityinwater Verylittleaggregation Rhodaminedyederivative MolarMass:611g/mol ATTO565Datasheet
OpticalProperties: λex =563nm
λem =592nm
εmax =1.2×105
Φf = 0.9
τfl =3.4n
Brightness =10
Laser =532nm
Filterset = TRITC
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
![StressMarq/抗TASK1钾通道抗体[S374-48]/SMC-473D-A565/100-µg](https://www.ebiomall.cn/images/StressMarq/201710/SMC-473-TASK1-Potassium-Channel-Antibody-S374-48-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1-150x150.png)
