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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/抗TARP伽玛2/4/8(Stargazin)抗体[S245-36]/SMC-422D-A594/100-µg
商品详细StressMarq/抗TARP伽玛2/4/8(Stargazin)抗体[S245-36]/SMC-422D-A594/100-µg
StressMarq/抗TARP伽玛2/4/8(Stargazin)抗体[S245-36]/SMC-422D-A594/100-µg
StressMarq/抗TARP伽玛2/4/8(Stargazin)抗体[S245-36]/SMC-422D-A594/100-µg
商品编号: SMC-422D-A594
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name TARP Gamma2/4/8 (Stargazin) Antibody
Description

Mouse Anti-Rat TARP Gamma2/4/8 (Stargazin) Monoclonal IgG2A

Species Reactivity Human, Mouse, Rat
Applications WB, ICC/IF
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S245-36
Isotype IgG2a
Specificity Detects ~35-55kDa. Does not cross-react with TARPGamma3.
Cite This Product StressMarq Biosciences Cat# SMC-422, RRID: AB_2701343
Certificate of Analysis 1 µg/ml of SMC-422 was sufficient for detection of TARP Gamma2/4/8 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names CACNG2 Antibody, Calcium channel voltage dependent gamma subunit 2 Antibody, CaV gamma 2 Antibody, Ipr328 Antibody, MGC123981 Antibody, MGC138502 Antibody, MGC138504 Antibody, Voltage-dependent calcium channel gamma-2 subunit Antibody, Neuronal voltage-gated calcium channel gamma-2 subunit Antibody, Stargazin Antibody, Stg Antibody, Stargazer Antibody, TARP Antibody, TARP gamma-2 Antibody, Transmembrane AMPAR regulatory protein gamma-2 Antibody, AW060990 Antibody, B230105C07Rik Antibody, B930041E13Rik Antibody, Wag Antibody, Waggler Antibody
Research Areas Calcium Channels, Cell Markers, Cell Structure, Ion Channels, Neuron Markers, Neuroscience, Post-Synaptic Markers, Postsynaptic Markers, Voltage-Gated Calcium Channels
Cellular Localization Membrane
Accession Number NP_445803.1
Gene ID 84347
Swiss Prot Q71RJ2
Scientific Background Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
References 1. Gill M.B., et al. (2011) J Neurosci. 31(18): 6928-6938.
2. Yu L. (2011) Neuroscience. 178: 13-20.
3. Steinmetz C.C., Turrigiano G.G. (2010) J Neurosci. 30: 14685-14690.
4. Kato. A.S. et al. (2007) J Neurosci. 27: 4969-4977.
5. Letts V.A., et al. (1998) Nat Genet. 19: 340-34

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (SMC-422). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (SMC-422) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (SMC-422). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (SMC-422) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.

<p>Western Blot analysis of Rat brain lysates showing detection of Stargazin Calcium Channel protein using Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody, Clone S245-36 (SMC-422). Primary Antibody: Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody (SMC-422) at 1:100, 1:250, 1:500, and 1:1000.</p>

Western Blot analysis of Rat brain lysates showing detection of Stargazin Calcium Channel protein using Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody, Clone S245-36 (SMC-422). Primary Antibody: Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody (SMC-422) at 1:100, 1:250, 1:500, and 1:1000.

Product Citations (1)

Western Blot

Coexpressed Auxiliary Subunits Exhibit Distinct Modulatory Profiles on AMPA Receptor Function.

Khodosevich, K. et al. (2014) Neuron. 83(3):601-15.

PubMed ID: 25066086 Reactivity Mouse Applications: Western Blot

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。