Overview:
Product Name | Sodium-Iodide Symporter Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Human Sodium-Iodide Symporter Monoclonal IgG1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, AM | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Mannose binding protein hNIS fusion (AA468-643) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | 14F |
Isotype | IgG1 |
Specificity | Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-390, RRID: AB_10804544 |
Certificate of Analysis | 1 µg/ml of SMC-390 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | NIS Antibody, SLC5A5 Antibody, solute carrier family 5 Antibody, Na (+)I(-) cotransporter Antibody |
Research Areas | Cancer, Cell Signaling, Ion Pumps/Transporters, Neuroscience, Pumps/Transporters |
Cellular Localization | Membrane |
Accession Number | NP_000444.1 |
Gene ID | 6528 |
Swiss Prot | Q92911 |
Scientific Background | The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). |
References |
1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255. |
Product Images
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-390). Tissue: Thyroid . Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-390) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-390). Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-390) at 1:1000.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-390). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-390) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-390). Tissue: Thyroid . Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-390) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-390). Tissue: Thyroid . Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-390) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Product Citations (2)
Other Citations
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Human Applications: Antibody Microarray
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray
ATTO 565 | ||
Overview:
ATTO 565 Datasheet | Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |