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StressMarq/Anti-SOD (EC) Antibody [4GG11G6]/SMC-167D-A633/100-µg
Overview:
Product Name | SOD (EC) Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Human SOD (EC) Monoclonal IgG1 Kappa | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat, Guinea Pig (Cavia porcellus) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF, ELISA | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Human extracellular SOD purified from aortas | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | 4GG11G6 |
Isotype | IgG1 Kappa |
Specificity | Detects extracellular SOD ~35kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-167, RRID: AB_2191964 |
Certificate of Analysis | 1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | EC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody |
Research Areas | Cancer, Oxidative Stress |
Cellular Localization | Extracellular Space |
Accession Number | NP_003093.2 |
Gene ID | 6649 |
Swiss Prot | P08294 |
Scientific Background | Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1). |
References |
1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491 |
Product Images
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000.
Western Blot analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..
Product Citations (2)
Western Blot
Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans.
Konior, A. et al. (2011) J Mol Cel Cardiology. 50 (4): 686-694.
PubMed ID: 21112335 Reactivity Guinea-pig Applications: Western Blot
Immunohistochemistry
Endogenous enzymes (NOX and ECSOD) regulate smoke-induced oxidative stress.
Tollefson, A.K. et al. (2010) Free Radic Biol Med. 49 (12): 1937-1946.
PubMed ID: 20887783 Reactivity Human Applications: Immunohistochemistry
ATTO 633 | ||
Overview:
ATTO 633 Datasheet | Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |