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StressMarq/Anti-SHANK (pan) Antibody [S23b-49]/SMC-327D-A633/100-µg
Overview:
Product Name | SHANK (pan) Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Rat SHANK (pan) Monoclonal IgG1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, IP, ICC/IF, AM | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Fusion protein amino acids 84-309 of rat Shank2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | S23b-49 |
Isotype | IgG1 |
Specificity | Detects ~160kDa. Recognizes Shank1, 2 and 3. |
Cite This Product | StressMarq Biosciences Cat# SMC-327, RRID: AB_2301756 |
Certificate of Analysis | 1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | Cortactin binding protein 1 Antibody, Cortactin SH3 domain-binding protein Antibody, Cortactin-binding protein 1 Antibody, CortBP1 Antibody, CTTNBP1 Antibody, GKAP/SAPAP interacting protein Antibody, GKAP/SAPAP-interacting protein Antibody, KIAA1022 Antibody, Proline rich synapse associated protein 1 Antibody, Proline-rich synapse-associated protein 1 Antibody, ProSAP1 Antibody, SH3 and multiple ankyrin repeat domains protein 2 Antibody, SHAN2_RAT Antibody, SHANK Antibody, Shank2 Antibody, SPANK-3 Antibody |
Research Areas | Cell Markers, Cell Signaling, Cell Structure, Neuron Markers, Neuroscience, Post-Synaptic Markers, Postsynaptic Markers, Scaffold Proteins, Scaffolds |
Cellular Localization | Cytoplasm, Cell Junction, Cell projection, Growth cone, Postsynaptic cell membrane, Postsynaptic density, Synapse |
Gene ID | 171093 |
Swiss Prot | Q9QX74 |
Scientific Background | Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer’s disease (3). |
References |
1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198. |
Product Images
Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (SMC-327). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (SMC-327) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (SMC-327). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (SMC-327) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.
Product Citations (3)
Western Blot
Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice.
Nagura, H. et al. (2012) Mol Brain. 5 (43).
PubMed ID: 23268962 Reactivity Mouse Applications: Western Blot
Other Citations
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Human Applications: Antibody Microarray
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray
ATTO 633 | ||
Overview:
ATTO 633 Datasheet | Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |