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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > 应激MARQ/抗SAPAP(pan)抗体[S127-31]/SMC-338D-A594/100-µg
商品详细应激MARQ/抗SAPAP(pan)抗体[S127-31]/SMC-338D-A594/100-µg
应激MARQ/抗SAPAP(pan)抗体[S127-31]/SMC-338D-A594/100-µg
应激MARQ/抗SAPAP(pan)抗体[S127-31]/SMC-338D-A594/100-µg
商品编号: SMC-338D-A594
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name SAPAP (pan) Antibody
Description

Mouse Anti-Rat SAPAP (pan) Monoclonal IgG2b

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, IP, AM
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S127-31
Isotype IgG2b
Specificity Detects ~120kDa (SAPAP2), ~110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs.
Cite This Product StressMarq Biosciences Cat# SMC-338, RRID: AB_2092531
Certificate of Analysis 1 µg/ml of SMC-338 was sufficient for detection of SAPAP 1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names DAP 1 ALPHA antibody, DAP 1 antibody, DAP 1 BETA antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, DAP1 antibody, Discs large (Drosophila) homolog associated protein 1 antibody, Discs large homolog associated protein 1 antibody, Disks large associated protein 1 antibody, Disks large associated protein 1 alpha isoform included antibody, Disks large associated protein 1, beta isoform, included antibody, Disks large-associated protein 1 antibody, DLGAP 1 antibody, Dlgap1 antibody, DLGP1_HUMAN antibody GKAP antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Guanylate kinase associated protein antibody, Guanylate kinase-associated protein antibody, hGKAP antibody, KIAA4162 antibody, MGC88156 antibody, mKIAA4162 antibody, PSD 95/SAP90 binding protein 1 antibody, PSD-95/SAP90-binding protein 1 antibody, SAP90/PSD 95 associated protein 1 antibody, SAP90/PSD-95-associated protein 1 antibody SAPAP 1 antibody, SAPAP1 antibody
Research Areas Cell Markers, Cell Structure, Neuron Markers, Neuroscience, Post-Synaptic Markers, Postsynaptic Markers
Cellular Localization Cell Junction, Cell membrane, Postsynaptic cell membrane, Postsynaptic density, Synapse
Accession Number NP_075235.2
Gene ID 65040
Swiss Prot P97836
Scientific Background The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components (1, 2). SAPAP1, 2 and 4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies (1, 3).
References 1. Kindler S., et al. (2004) Brain Res. Mol Brain Res. 126: 14-21.
2. Bongiorno-Borbone L., et al. (2005) Biochem, Biophys. Res Commun. 337: 641-646.
3. Welch J.M., Wang D., and Fend G. (2004) J Comp. Neurol. 472: 24-39.

Product Images

<p>Western Blot analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 (SMC-338). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody (SMC-338) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.</p>

Western Blot analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 (SMC-338). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody (SMC-338) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Citations (2)

Other Citations

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Human Applications: Antibody Microarray

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。