Overview:
Product Name | SAPAP (pan) Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Rat SAPAP (pan) Monoclonal IgG2b | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF, IP, AM | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | S127-31 |
Isotype | IgG2b |
Specificity | Detects ~120kDa (SAPAP2), ~110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs. |
Cite This Product | StressMarq Biosciences Cat# SMC-338, RRID: AB_2092531 |
Certificate of Analysis | 1 µg/ml of SMC-338 was sufficient for detection of SAPAP 1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | DAP 1 ALPHA antibody, DAP 1 antibody, DAP 1 BETA antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, DAP1 antibody, Discs large (Drosophila) homolog associated protein 1 antibody, Discs large homolog associated protein 1 antibody, Disks large associated protein 1 antibody, Disks large associated protein 1 alpha isoform included antibody, Disks large associated protein 1, beta isoform, included antibody, Disks large-associated protein 1 antibody, DLGAP 1 antibody, Dlgap1 antibody, DLGP1_HUMAN antibody GKAP antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Guanylate kinase associated protein antibody, Guanylate kinase-associated protein antibody, hGKAP antibody, KIAA4162 antibody, MGC88156 antibody, mKIAA4162 antibody, PSD 95/SAP90 binding protein 1 antibody, PSD-95/SAP90-binding protein 1 antibody, SAP90/PSD 95 associated protein 1 antibody, SAP90/PSD-95-associated protein 1 antibody SAPAP 1 antibody, SAPAP1 antibody |
Research Areas | Cell Markers, Cell Structure, Neuron Markers, Neuroscience, Post-Synaptic Markers, Postsynaptic Markers |
Cellular Localization | Cell Junction, Cell membrane, Postsynaptic cell membrane, Postsynaptic density, Synapse |
Accession Number | NP_075235.2 |
Gene ID | 65040 |
Swiss Prot | P97836 |
Scientific Background | The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components (1, 2). SAPAP1, 2 and 4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies (1, 3). |
References |
1. Kindler S., et al. (2004) Brain Res. Mol Brain Res. 126: 14-21. 2. Bongiorno-Borbone L., et al. (2005) Biochem, Biophys. Res Commun. 337: 641-646. 3. Welch J.M., Wang D., and Fend G. (2004) J Comp. Neurol. 472: 24-39. |
Product Images
Western Blot analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 (SMC-338). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody (SMC-338) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Citations (2)
Other Citations
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Human Applications: Antibody Microarray
Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.
Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.
PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray
ATTO 594 | ||
Overview:
ATTO 594 Datasheet | Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |