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StressMarq/StressXpress® Retinol Binding Protein Urinary EIA Kit/SKT-218-96/96-well
StressMarq/StressXpress® Retinol Binding Protein Urinary EIA Kit/SKT-218-96/96-well
商品编号：
SKT-218-96
市场价：
¥9140.00
美元价：
5484.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | RetinolBindingProteinUrinaryEIAKit |
| Description | ColorimetricmeasurementofRBPlevelsinurine |
| SpeciesReactivity | Dog,Human,Monkey,Rat |
| Platform | Microplate |
| SampleTypes | Urine |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichEIA(EnzymeImmunoassay) |
| Utility | EIAkitusedtomeasureRBPlevelsinurinesamples. |
| Sensitivity | 2.90ng/ml |
| AssayRange | 3.906-1000ng/ml |
| Precision | IntraAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninreplicatesof8inanassay.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.8ng/mL,4.5%CVSample2-28.0ng/mL,7.5%CVSample3-53.5ng/mL,7.3%CVSample4-323.7ng/mL,2.1%CVInterAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.4ng/mL,9.1%CVSample2-28.0ng/mL,9.2%CVSample3-50.8ng/mL,8.1%CVSample4-309.2ng/mL,14.0%CV |
| IncubationTime | 90Minutes |
| NumberofSamples | 41samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||
| ProductType | EIAKits | ||||||||||||||||||||||||||||||
| AssayOverview | TheRetinolBindingProteinUrinaryEIAKitisdesignedtoquantitativelymeasureRBPpresentinurinesamples.ARBPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.ARBP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionoftheRBPpolyclonalantibodytoeachwell.Afteranhourincubationtheplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundRBP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheRBPinthesampleiscalculated,aftermakingasuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | ||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | RetinolBindingProteinUrinaryEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-218) |
BIOLOGicalDescription
| AlternativeNames | CellularretinolbindingproteinEIAKit,CRBPEIAKit,CRBPEIAKit,CRBP1EIAKit,CRBP2EIAKit,RBP1EIAKit,RBP2EIAKit,RBP4EIAKit,RBPCEIAKit,Retinolbindingprotein1EIAKit,Retinolbindingprotein2EIAKit,Retinolbindingprotein4EIAKit |
| ResearchAreas | CardiovascularSystem,CellSignaling |
| ScientificBackground | Retinolbindingprotein(RBP)isfromafamilyofstructurallyrelatedproteinsthatbindsmallhydrophobicmoleculessuchasbilepigments,steroids,odorants,etc(1).RBPisa21kDahighlyconserved,single-chainglycoprotein,consistingof182aminoacidswith3disulfidebonds,thathasahydrophobicpocketwhichbindsretinol(vitaminA).RBPbindsretinolina1:1stoichiometry,whichservestonotonlysolubilizeretinolbutalsoprotectitfromoxidation.Wheninserum,themajorityofRBPboundwithretinolisreversIBLycomplexedwithtransthyretin(prealbumin)(2,3).Thiscomplexthentransportsretinoltospecificreceptorsofvarioustissuesinthebody.VitaminAstatusisreflectedbyserumconcentrationasitishemostaticallycontrolledanddoesnotfalluntilstoresaredramaticallyreduced(4,5).RBPhasalsobeenshowntobeausefulMarkerforrenalfunction(6)asitistotallyfilteredbytheglomeruliandreabsorbedbyproximaltubules(7).ThishasmadeurinaryRBP(uRBP)atooltostudyrenalfunctioninheart(8)orkidney(9)transplantrecipients,type1and2diabetics(10),andinpeopleexposedtouraniumfromminingoperations(11).MeasurementofuRBPlevelshasalsobeenusefulindetectionandcharacterizationofdiseasesincludinghypertension(12)andcertaincancers(13,14). |
| References | 1.BlanerWS.EndocrRev.1989Aug;10(3):308–16. 2.WolfG.Physiol.Rev.1984Jul;64(3):873-937. 3.PetersenPA.J.Biol.Chem.1971,246:34-43. 4.GoodmanDS,BlanerWS.inTheRetinoids,eds.SpornMB,RobertsAB,GoodmanDS,(Orlando:AcademicPress,1984)vol.2,1-39. 5.Olson,JA.ModernNutritioninHealthandDisease,eds.ShilsME,OlsonJA,ShikeM,(Philadelphia:Lea&Febiger,1994)8thed.287–307. 6.PetersonPA,andBerggårdI.J.Biol.Chem.1971246:25-33. 7.BernardAM,andLauwerysRR.Clin.Chem.198127:1781-2. 8.CamaraNO,MatosAC,RodriguesDAetal.TheLancet2001357:856-857. 9.HosakaB,ParkSI,FelipeCR,GarciaRG,MachadoPG,PereiraAB,Tedesco-SilvaHandMedina-PestanaJO.TransplantProc.200335:1341-1343. 10.AbahusainMA,WrightJ,DickersonJWTanddeVolEB.AmJClinNutr.2004;79:218-25. 11.WyattSA,ReitzLV,CroleyTR,HawkinsD,BarrettE,McKeownA,PowellN,WestA,HamnerTandRoysterMO.JExpSciEnvironEpidem.200818:59-75. 12.BangLE,HolmJandSvendsenTL.Am.J.Hypertens19969:1024-1028. 13.MoiseAR,NoyN,PalczewskiK,BlanerWS.Biochemistry20079:1024-1028. 14.GavrilovV,YermiahuT,GorodischerR.KidneyInt.2006Jan;69(1):173-7. |
ProductImages
TypicalStandardCurvefortheRetinolBindingProteinUrinaryEIAKit(EnzymeImmunoassay)StressXpress®–SKT-218.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:3.906–1000ng/ml.
Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:2,onewithalowdilutedRBPlevelof14.5ng/mLandonewithahigherdilutedlevelof299.2ng/mLandmixingthemingivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
