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StressMarq/Anti-REEP1 Antibody [S345-51]/SMC-480D-A594/100-µg
Overview:
Product Name | REEP1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Mouse REEP1 Monoclonal IgG2B | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Fusion protein amino acids 111-201 of mouse REEP1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | S345-51 |
Isotype | IgG2b |
Specificity | Detects ~22kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-480, RRID: AB_2702351 |
Certificate of Analysis | A 1:100 dilution of SMC-480 was sufficient for detection of REEP1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | Receptor expression-enhancing protein 1 Antibody, C2orf23 Antibody, D6Ertd253e Antibody, rcG_56072 Antibody, Reep1 Antibody, SPG31 Antibody |
Research Areas | Cell Markers, Cell Structure, Membrane Markers, Neurodegeneration, Neuron Markers, Neuroscience, Spastic Paraplegia |
Cellular Localization | Endoplasmic Reticulum, Endoplasmic reticulum membrane, Mitochondrion, Mitochondrion membrane |
Accession Number | NP_848723.1 |
Gene ID | 52250 |
Swiss Prot | Q8BGH4 |
Scientific Background | REEP1 (receptor expression-enhancing protein 1), is a 201 amino acid multi-pass mitochondrion membrane protein that belongs to the DP1 family. REEP1 interacts with odorant receptor proteins and may enhance the cell surface expression of odorant receptors (1, 2). It is also required for endoplasmic-reticulum network formation shaping and remodeling by linking ER tubules to the cytoskeleton (2). Mutations in the REEP1 gene are a common cause of hereditary spastic paraplegia (HSP). Mutations in the REEP1 gene also cause spastic paraplegia autosomal dominant type 31, a neurodegenerative disorder (3). |
References |
1. Genes and mapped phenotypes. (n.d.). Retrieved March 11, 2015, from http://www.ncbi.nlm.nih.gov/gene/65055 2. Park S.H., Zhu P.P., Parker R.L. Blackstone C. (2010) J Clin Invest. 120(4): 1097-1110. 3. REEP1 - Receptor expression-enhancing protein 1 - Homo sapiens (Human). (n.d.). Retrieved March 11, 2015, from http://www.uniprot.org/uniprot/Q9H902 |
Product Images
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) REEP1 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~22 kDa REEP1 protein using Mouse Anti-REEP1 Monoclonal Antibody, Clone S345-51 (SMC-480). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1 Monoclonal Antibody (SMC-480) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~22 kDa.
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ATTO 594 | ||
Overview:
ATTO 594 Datasheet | Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |