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StressMarq/StressXpress® Rat GRP78 ELISA Kit/SKT-137-96/96-well
StressMarq/StressXpress® Rat GRP78 ELISA Kit/SKT-137-96/96-well
商品编号：
SKT-137-96
市场价：
¥11200.00
美元价：
6720.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | RatGRP78ELISAKit |
| Description | ColorimetricdetectionofGRP78inratlysates |
| SpeciesReactivity | Rat |
| Platform | Microplate |
| SampleTypes | Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateGRP78concentrationinratsamples. |
| Sensitivity | 0.15ng/ml |
| AssayRange | 0-50ng/ml |
| Precision | Intra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%. |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor2hours.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatat37°Cfor2hours.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidinPolyHRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateat37°Cfor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | RatGRP78ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-137) |
BIOLOGicalDescription
| AlternativeNames | Grp78ELISAKit,BindingimmunoglobulinproteinELISAKit,BiPELISAKit,78kDaglucose-regulatedproteinELISAKit,heatshock70kDaprotein5ELISAKit |
| ResearchAreas | Cancer,HeatShock,CellSignaling,Chaperones,OrganelleMarkers,Trafficking |
| ScientificBackground | GRP78isaubiquitouslyexpressed,78-kDaglucose-regulatedprotein,andiscommonlyreferredtoasanimmunoglobinchainbindingprotein(BiP).TheBiPproteinsarecategorizedasstressresponseproteinsbecausetheyplayanimportantroleintheproperfoldingandassemblyofnascentproteinandinthescavengingofmisfoldedproteinsintheendoplasmicreticulumlumen.TranslationofBiPisdirectedbyaninternalribosomalentrysite(IRES)inthe5’nontranslatedregionoftheBiPmRNA.BiPIRESactivityincreaseswhencellsareheatstressed(1).GRP78isalsocriticalformaintenanceofcellhomeostasisandthepreventionofapoptosis(2).Luoetal.haveprovidedfindingsthatsuggestGRP78isessentialforembryoniccellgrowthandpluripotentcellsurvival(3).Intermsofdiseases,GRP78hasbeenshowntobeareliablebiomarkerofhypoglycemia,toserveaneuroprotectivefunctioninneuronsexposedtoglutamateandoxidativestress(4),anditsproteinlevelsarereducedinthebrainsofAlzheimer’spatients(5).Also,theinductionoftheGRP78proteinthatresultsinsevereglucoseandoxygendeprivationcouldpossIBLeleadtodrugresistancetoantitumordrugs(6,7). |
| References | 1.ChoS.,etal.(2007).MolCellBiol27(1):368-83. 2.YangY.,etal.(1998)JBiolChem273:25552-25555. 3.LuoS.,etal(2006)26(15):5688-97. 4.YuZ.,etal.(1999)ExpNeurol.15:302-314. 5.KoomagiR.,etal.(1999)AnticancerRes.19:4333-4336. 6.LaquerreS.,etal.(1998)J.Virology72:4940-4949. 7.DongD.,etal.(2005)CancerRes65(13):5785-91. |
ProductImages
TypicalStandardCurvefortheRatGRP78ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-137.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0–50ng/ml.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
