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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-RAF1 Antibody (pSer296)/SPC-1071D-HRP/100-µl
商品详细StressMarq/Anti-RAF1 Antibody (pSer296)/SPC-1071D-HRP/100-µl
StressMarq/Anti-RAF1 Antibody (pSer296)/SPC-1071D-HRP/100-µl
StressMarq/Anti-RAF1 Antibody (pSer296)/SPC-1071D-HRP/100-µl
商品编号: SPC-1071D-HRP
市场价: ¥6600.00
美元价: 3960.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameRAF1Antibody(pSer296)
Description

RabbitAnti-HumanRAF1(pSer296)Polyclonal

SpeciesReactivityHuman
ApplicationsWB,AM
AntibodyDilutionWB(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenAphospho-specificpeptidecorrespondingtoresiduessurroundingSer296ofhumanRAF1(AA293-299)
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationPeptideAffinityPurified
ClonalityPolyclonal
SpecificityDetects~73kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-1071,RRID:AB_2709677
CertificateofAnalysisA1:250dilutionofSPC-1071wassufficientfordetectionofRAF1(pSer296)in10µgofHeLacelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamescRafAntibody,C-rafAntibody,C-Rafproto-oncogene,serine/threoninekinaseAntibody,CMD1NNAntibody,Craf1transforminggeneAntibody,cRafAntibody,Craf1transforminggeneAntibody,EC2.7.11.1Antibody,kinaseRaf1Antibody,Murinesarcoma3611oncogene1Antibody,NS5Antibody,OncogeneMILAntibody,OncogeneRAF1Antibody,OTTHUMP00000160218Antibody,OTTHUMP00000207813Antibody,OTTHUMP00000209389Antibody,Proteinkinaseraf1Antibody,Proto-oncogenec-RAFAntibody,Raf1Antibody,Raf1protooncogeneserine/threoninekinaseAntibody,RAFAntibody,Rafprotooncogeneserine/threonineproteinkinaseAntibody,RAFproto-oncogeneserine/threonine-proteinkinaseAntibody,RAF-1Antibody,RAF1Antibody,RAF1_HUMANAntibody,Similartomurineleukemiaviral(V-raf-1)oncogenehomolog1Antibody,TRANSFORMINGREPLICATION-DEFECTIVEMURINERETROVIRUS3611-MSVAntibody,vraf1murineleukemiaviraloncogenehomolog1Antibody,v-rafmurinesarcomaviraloncogenehomolog1Antibody,vraf1murineleukemiaviraloncogenehomolog1Antibody
CellularLocalizationCytoplasm,Cellmembrane,Mitochondrion,Nucleus
AccessionNumberNP_002871
GeneID5894
SwissProtP04049
ScientificBackgroundRAF1isaprotein-serine/threoninekinase.RAF-1isupregulatedinmanycancerssinceRas(anupstreamactivatorofRAF)ismutated/upreguatedinmanycancers.Forexample,RAF-1isover-expressedduetooncogenicrasmutationsinabout35%ofNon-SmallCellLungCancer(NSCLC).AsmallnumberofRAFmutationshavebeenfoundinstomachandmouthtumours.RAF-1geneamplificationwasdetectedin4%ofbladdercancersamples,whiledeletionsattheRAF-1locusweredetectedin2.2%ofthesesamples;theseamplificationsanddeletionswerecorrelatedwithtumourgrade.

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HRP(HorserADIshperoxidase)

Properties:

  • Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
  • Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
  • Catalyzestheconversionof:
    • Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
    • Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
    • Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
  • Highturnoverrateenablesrapidgenerationofastrongsignal
  • 44kDaglycoprotein
  • Extinctioncoefficient:100(403nm)
  • Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。