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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-PUMA (NT) Antibody/SPC-165D-A565/100-µg
商品详细StressMarq/Anti-PUMA (NT) Antibody/SPC-165D-A565/100-µg
StressMarq/Anti-PUMA (NT) Antibody/SPC-165D-A565/100-µg
StressMarq/Anti-PUMA (NT) Antibody/SPC-165D-A565/100-µg
商品编号： SPC-165D-A565
市场价： ¥6820.00
美元价： 4092.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name PUMA (NT) Antibody
Description

Rabbit Anti-Human PUMA (NT) Polyclonal

Species Reactivity Human, Rat, Rainbow Trout (Oncorhynchus mykiss)
Applications WB, IHC
Antibody Dilution WB (1:500), IHC (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen N-terminal amino acids of human PUMA
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Cite This Product StressMarq Biosciences Cat# SPC-165, RRID: AB_2064544
Certificate of Analysis 2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Research Areas Cancer, Apoptosis
Cellular Localization Mitochondrion
Accession Number NP_001120712.1
Gene ID 27113
Swiss Prot Q9BXH1
Scientific Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References 1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94.
2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82.
3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:100.</p>

Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:100.

<p>Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:500.</p>

Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:500.

<p>Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.</p>

Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Product Citations (2)

Immunohistochemistry

Prolonged Expression of Puma in Cholinergic Amacrine Cells During the Development of Rat Retina.

Wakabayashi, T., Kosaka, J., Mori, T. and Yamada, H. (2012) J Histochem Cytochem. 60 (10): 777-788.

PubMed ID: 22736709 Reactivity Rat Applications: Immunohistochemistry

Other Citations

Plasma exosomes are enriched in Hsp70 and modulated by stress and cortisol in rainbow trout.

Faught, E., Henrickson, L. and Vijayan, M.M. (2016) J Endocrinol. 232(2):237-246.

PubMed ID: 27872197 Reactivity Rainbow Trout

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

 ATTO 565 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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