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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > STRESS MARQ/抗PUMA(CT)抗体/SPC-166D-A594/100-微克
商品详细STRESS MARQ/抗PUMA(CT)抗体/SPC-166D-A594/100-微克
STRESS MARQ/抗PUMA(CT)抗体/SPC-166D-A594/100-微克
STRESS MARQ/抗PUMA(CT)抗体/SPC-166D-A594/100-微克
商品编号: SPC-166D-A594
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name PUMA (CT) Antibody
Description

Rabbit Anti-Human PUMA (CT) Polyclonal

Species Reactivity Human, Mouse
Applications WB, ICC/IF
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen C-terminal amino acids of human PUMA
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Cite This Product StressMarq Biosciences Cat# SPC-166, RRID: AB_2064543
Certificate of Analysis 1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Research Areas Cancer, Apoptosis
Cellular Localization Mitochondrion
Accession Number NP_001120712.1
Gene ID 27115
Swiss Prot Q9BXH3
Scientific Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References 1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94.
2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82.
3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

<p>Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:1000.</p>

Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:1000.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:100.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:100.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。