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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/抗原钙粘蛋白γA3抗体[S144-17]/SMC-451D-A594/100-µg
商品详细StressMarq/抗原钙粘蛋白γA3抗体[S144-17]/SMC-451D-A594/100-µg
StressMarq/抗原钙粘蛋白γA3抗体[S144-17]/SMC-451D-A594/100-µg
StressMarq/抗原钙粘蛋白γA3抗体[S144-17]/SMC-451D-A594/100-µg
商品编号: SMC-451D-A594
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Protocadherin Gamma A3 Antibody
Description

Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1

Species Reactivity Human, Mouse
Applications WB, ICC/IF
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Mouse
Immunogen Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A protein.
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S144-17
Isotype IgG1
Specificity Detects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
Cite This Product StressMarq Biosciences Cat# SMC-451, RRID: AB_2701865
Certificate of Analysis 1 µg/ml of SMC-451 was sufficient for detection of Protocadherin Gamma A3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma A3) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names PCDHGA3 Antibody, PCDG3 Antibody, PCDH gamma A3 Antibody, Protocadherin gamma subfamily A 3 Antibody, Protocadherin gamma-A3 Antibody, Gamma Protocadherin-A3 Antibody, Gamma-Protocadherin-A3 Antibody
Research Areas Cell Markers, Cell Structure, Membrane Markers, Neuron Markers, Neuroscience
Cellular Localization Cell membrane
Accession Number NP_291064.1
Gene ID 93711
Swiss Prot Q91XY5
Scientific Background Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHGA3 (Protocadherin gamma-A3) is a 932 amino acid that is one of 22 proteins encoded by the protocadherin gamma cluster. The protocadherein gamma cluster consists of three subfamilies (A, B and C) and PCDHGA3 is a member of the gamma subfamily A. PCDHGA3 is a type I transmembrane receptor containing six cadherin motifs and is expressed in the central nervous system where it localizes to synapses. Members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGA3 that are produced as a result of alternative splicing events.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite.

<p>Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.</p>

Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。