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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/StressXpress® Prostaglandin E2 EIA Kit/SKT-207-96/96-well
商品详细StressMarq/StressXpress® Prostaglandin E2 EIA Kit/SKT-207-96/96-well
StressMarq/StressXpress® Prostaglandin E2 EIA Kit/SKT-207-96/96-well
StressMarq/StressXpress® Prostaglandin E2 EIA Kit/SKT-207-96/96-well
商品编号: SKT-207-96
市场价: ¥9140.00
美元价: 5484.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameProstaglandinE2EIAKit
Description

QuantitativecolorimetricmeasurementofPGE2

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesEDTAPlasma,HeparinPlasma,Saliva,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtoquantitativelymeasurePGE2presentinsamples.
Sensitivity3.07pg/ml
AssayRangeHS:1.953-500pg/ml.LV:15.625-1000pg/ml.RG:3.906-500pg/ml.ON:3.906-500pg/ml.
PrecisionIntraAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedProstaglandinE2concentrationswere:Sample1-11.7pg/mL,12.3%CVSample2-98.6pg/mL,6.3%CVSample3-131.2pg/mL,4.9%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesinseventeenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedProstaglandinE2concentrationswere:Sample1-12.3pg/mL,8.8%CVSample2-100.5pg/mL,8.1%CVSample3-134.7pg/mL,9.8%CV
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS,SalivaSampleHandlingInstructions,EicosanoidSampleExtractionProtocol

Properties

StorageTemperature-20ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheProstaglandinE2(PGE2)EIAkitisdesignedtoquantitativelymeasurePGE2presentinserum,plasma,urine,saliva,cells,tissue,andtissueculturemediasamples.ThisEIAkitallowsforthewidestvariationsinsamplesize,sensitivityandassaytimingofanyPGE2kit.Theprotocolvariationsareoutlinedbelow:TheRegularFormat,whichuses50µLofsampleorstandard,isshownonpages10-11,givesresultsin2.5hours.TheLowSampleVolumeFormat,whichuses25µLofsampleorstandard,isoutlinedonpage12-13,alsogivesresultsin2.5hours,butuseslowersamplevolumes.TheHighSensitivityFormat,whichuses100µLofsampleorstandard,isshownonpage14-15,alsogivesresultsin2.5hours,butisthehighestsensitivitykitofanytypeavailable.TheOvernightFormat,whichuses50µLofsampleorstandard,isshownonpage16-17,andusesaprimaryovernightincubationat4°Ctofityourworkflow.ThisformathassimilarsensitivitytotheRegularFormatoutlinedabove.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-207ACoatedWhite96WellPlates1Each
SKC-207BProstaglandinE2Standard70µl
SKC-207CStressXpress®ProstaglandinE2Antibody3ml
SKC-207DStressXpress®ProstaglandinE2Conjugate3ml
SKC-207EAssayBuffer28ml
SKC-207FWashBufferConcentrate30ml
SKC-207GTMBSolution11ml
SKC-207HStopSolution5ml
SKC-207IPlateSealer1Each
CiteThisProductProstaglandinE2EIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-207)

BIOLOGicalDescription

AlternativeNames(5Z,11α,13E,15S)-7-[3-hydroxy-2-(3-hydroxyoct-1-enyl)-5-oxo-cyclopentyl]hept-5-enoicacidEIAKit
ResearchAreasCancer,CardiovascularSystem,CellSignaling
ScientificBackgroundEicosanoidsignaltransductionpathwaysarehighlyconservedandareinvolvedinanumberofphysiologicalprocesses.Prostaglandinsaresynthesizedfromarachidonicacidbycyclooxygenase(COX)-1or-2,whichconverttheacidintoPGH2.ThisisfurtherprocessedbycytosolicormicrosomalprostaglandinsynthasestobecomePGE2oroneofseveralotherprostanoids(1-3).ProstacyclinisthemajorcyclooxygenaseproductinbloodvesselwallsanditispresentininflammatoryfluidsinsimilarconcentrationstoPGE2.ProstacyclinisapotentvasodilatorandismorepotentthanPGE2inproducinghyperalgesia(4).PGE2isproducedbyawidevarietyoftissues(5-12)andinseveralpathologicalconditions,includinginflammation,arthritis,fever,tissueinjury,endometriosis,andavarietyofcancers(5,6).OtherbiologicalactionsofPGE2includevasodilation,modulationofsleep/wakecycles,andfacilitationofhumanimmunodeficiencyvirusreplication.ItelevatescAMPlevels,stimulatesboneresorption,andhasThermoregulatoryeffects.Ithasbeenshowntobearegulatorofsodiumexcretionandrenalhemodynamics(7-12).
References1.Moncada,S.,Ferriera,SH.&Vane,JR.(1979).HandbookofExperimentalPharmacology,50/II.Pp.588-616.Vane,J.R.&Ferreira,S.H.Berlin,NewYork:Springer.
2.Vane,JR.(1971).Nature,231,232-235.3.
3.Willis,AL.(1969).InProstaglandins,PeptidesandAmines.Pp.31-38.Ed.Mantegazza,P.&Horton,E.W.London:AcademicPress.
4.Higgs,GA.,Cardinal,DC.,Moncada,S.&Vane,JR.(1979).MicrovascularRes.,18,245-254.
5.Kargman,S.etal.(1996)BiochemPharmacol.52(7):1113-25
6.ThunMJ,NamboodiriMM,HeathCWJr.NewEngl.J.Med.(1991)325:1593–6.
7.RichardsonPDandWithringtonPG.Brit.J.Pharmacol.(1976)57:581-588.
8.O.Hayaishi.J.Biol.Chem.(1988)263:14593-14596.
9.S.Kuno,etal.Proc.Natl.Acad.Sci.,USA,(1986)83:3487-3490.
10.D.L.Bareis,etal.Proc.Natl.Acad.Sci.,USA,(1983)80:2514-2518.
11.L.G.Raisz,etal.Nature.(1977)267:532-534.
12.C.R.Long,KinoshitaY,KnoxFG.Prostaglandins.(1990)40:591-601.

ProductImages

<p>Regular Format Typical Standard Curve for the Prostaglandin E2 EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-207. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: HS: 1.953 – 500 pg/ml. LV: 15.625 – 1000 pg/ml. RG: 3.906 – 500 pg/ml. ON: 3.906 – 500 pg/ml. .</p>

RegularFormatTypicalStandardCurvefortheProstaglandinE2EIAKit(EnzymeImmunoassay)StressXpress®–SKT-207.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:HS:1.953–500pg/ml.LV:15.625–1000pg/ml.RG:3.906–500pg/ml.ON:3.906–500pg/ml..

<p>Multi-Format Standard Curve for the Prostaglandin E2 EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-207. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: HS: 1.953 – 500 pg/ml. LV: 15.625 – 1000 pg/ml. RG: 3.906 – 500 pg/ml. ON: 3.906 – 500 pg/ml. .</p>

Multi-FormatStandardCurvefortheProstaglandinE2EIAKit(EnzymeImmunoassay)StressXpress®–SKT-207.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:HS:1.953–500pg/ml.LV:15.625–1000pg/ml.RG:3.906–500pg/ml.ON:3.906–500pg/ml..

<p>Linearity was determined in human plasma and urine samples by taking two diluted samples with known PGE2 concentrations. A plasma sample with a high PGE2 concentration of 216.4 pg/mL was mixed with one with a lower value of 42.5 pg/mL. A urine sample with a high PGE2 concentration of 32.6 pg/mL was mixed with one with a lower value of 8.6 pg/mL. They were mixed in the ratios. The measured concentrations were compared to the expected values based on the ratios used.</p>

LinearitywasdeterminedinhumanplasmaandurinesamplesbytakingtwodilutedsampleswithknownPGE2concentrations.AplasmasamplewithahighPGE2concentrationof216.4pg/mLwasmixedwithonewithalowervalueof42.5pg/mL.AurinesamplewithahighPGE2concentrationof32.6pg/mLwasmixedwithonewithalowervalueof8.6pg/mL.Theyweremixedintheratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。