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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl
商品详细StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl
StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl
StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl
商品编号: SPC-1309D-A700
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNamePPARalphaAntibody
Description

RabbitAnti-HumanPPARalphaPolyclonal

SpeciesReactivityHuman
ApplicationsWB,ICC/IF,ELISA
AntibodyDilutionWB(1:1000),ICC/IF(1:500),ELISA(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedunphosphorylatedpeptidederivedfromhumanPPARαaroundthephosphorylationsiteofserine21(L-E-SP-P-L).
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~52kDa,endogenouslevelsoftotalPPARαprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1309,RRID:AB_2713349
CertificateofAnalysisA1:1000dilutionofSPC-1309wassufficientfordetectionofPPARalphain10µgofNIH/3T3celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNameshPPARAntibody,MGC2237Antibody,MGC2452Antibody,NR1C1Antibody,Nuclearreceptorsubfamily1groupCmember1Antibody,OTTHUMP00000197740Antibody,OTTHUMP00000197741Antibody,PeroxisomeproliferativeactivatedreceptoralphaAntibody,PeroxisomeproliferatoractivatedreceptoralphaAntibody,Peroxisomeproliferator-activatedreceptoralphaAntibody,PPARAntibody,PPAR-alphaAntibody,pparaAntibody,PPARA_HUMANAntibody,PPARalphaAntibody
CellularLocalizationNucleus
AccessionNumberNP_005027.2
GeneID5465
SwissProtQ07869
ScientificBackgroundPeroxisomeproliferatorsarenongenotoxiccarcinogenswhicharepurportedtoexerttheireffectoncellsthroughtheirinteractionwithmembersofthenuclearhormonereceptorfamilytermedperoxisomeproliferatoractivatedreceptors(PPARs).NuclearhormonereceptorsareliganddependentintracellularproteinsthatstimulatetranscriptionofspecificgenesbybindingtospecificDNAsequencesfollowingactivationbytheappropriateligand.PPARGismainlypresentinADIposetissue,colonandmacrophages.TwoisoformsofPPARGaredetectedinthehumanandinthemouse:PPAR-γ1(foundinnearlyalltissuesexceptmuscle)andPPAR-γ2(mostlyfoundinadiposetissueandtheintestine)
References1.Chandra,V.etal.(2008)Nature456(7220):350–356.
2.GreeneMEetal.(1995)GeneExpr.4(4–5):281–99.
3.ElbrechtA.etal.(1996)Biochem.Biophys.Res.Commun.224(2):431–7.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309).Tissue:HeLa.Species:Human.PrimaryAntibody:RabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~52kDa PPAR alpha protein using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:1000. Predicted/Observed Size: ~52kDa.</p>

WesternblotanalysisofMouseNIH/3T3celllysatesshowingdetectionof~52kDaPPARalphaproteinusingRabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309).Lane1:MouseNIH/3T3.Lane2:MouseNIH/3T3treatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309)at1:1000.Predicted/ObservedSize:~52kDa.

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 ATTO700
Overview:
  • Highfluorescenceyield
  • ExcellentthermalandphotostABIlity
  • Quenchedbyelectrondonors
  • Veryhydrophilic
  • Goodsolubilityinpolarsolvents
  • Zwitterionicdye
  • MolarMass:575g/mol

ATTO700Datasheet

 ATTO 700 Fluorophore Absorption and Emission SpectrumOpticalProperties:

λex =700 nm

λem=719nm

εmax=1.25×105

Φf= 0.25

τfl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。