StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl

StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A700/100-µl

商品编号： SPC-1309D-A700

品牌： StressMarq Biosciences

市场价： ¥6040.00

美元价： 3624.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName PPARalphaAntibody

Description

RabbitAnti-HumanPPARalphaPolyclonal

SpeciesReactivity Human

Applications WB,ICC/IF,ELISA

AntibodyDilution WB(1:1000),ICC/IF(1:500),ELISA(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen SynthesizedunphosphorylatedpeptidederivedfromhumanPPARαaroundthephosphorylationsiteofserine21(L-E-SP-P-L).

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	AffinityPurified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects~52kDa,endogenouslevelsoftotalPPARαprotein.
CiteThisProduct	StressMarqBiosciencesCat#SPC-1309,RRID:AB_2713349
CertificateofAnalysis	A1:1000dilutionofSPC-1309wassufficientfordetectionofPPARalphain10µgofNIH/3T3celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	hPPARAntibody,MGC2237Antibody,MGC2452Antibody,NR1C1Antibody,Nuclearreceptorsubfamily1groupCmember1Antibody,OTTHUMP00000197740Antibody,OTTHUMP00000197741Antibody,PeroxisomeproliferativeactivatedreceptoralphaAntibody,PeroxisomeproliferatoractivatedreceptoralphaAntibody,Peroxisomeproliferator-activatedreceptoralphaAntibody,PPARAntibody,PPAR-alphaAntibody,pparaAntibody,PPARA_HUMANAntibody,PPARalphaAntibody
CellularLocalization	Nucleus
AccessionNumber	NP_005027.2
GeneID	5465
SwissProt	Q07869
ScientificBackground	Peroxisomeproliferatorsarenongenotoxiccarcinogenswhicharepurportedtoexerttheireffectoncellsthroughtheirinteractionwithmembersofthenuclearhormonereceptorfamilytermedperoxisomeproliferatoractivatedreceptors(PPARs).NuclearhormonereceptorsareliganddependentintracellularproteinsthatstimulatetranscriptionofspecificgenesbybindingtospecificDNAsequencesfollowingactivationbytheappropriateligand.PPARGismainlypresentinADIposetissue,colonandmacrophages.TwoisoformsofPPARGaredetectedinthehumanandinthemouse:PPAR-γ1(foundinnearlyalltissuesexceptmuscle)andPPAR-γ2(mostlyfoundinadiposetissueandtheintestine)
References	1.Chandra,V.etal.(2008)Nature456(7220):350–356. 2.GreeneMEetal.(1995)GeneExpr.4(4–5):281–99. 3.ElbrechtA.etal.(1996)Biochem.Biophys.Res.Commun.224(2):431–7.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309).Tissue:HeLa.Species:Human.PrimaryAntibody:RabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~52kDa PPAR alpha protein using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:1000. Predicted/Observed Size: ~52kDa.</p>

WesternblotanalysisofMouseNIH/3T3celllysatesshowingdetectionof~52kDaPPARalphaproteinusingRabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309).Lane1:MouseNIH/3T3.Lane2:MouseNIH/3T3treatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-PPARalphaPolyclonalAntibody(SPC-1309)at1:1000.Predicted/ObservedSize:~52kDa.

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ATTO700

Overview:

Highfluorescenceyield
ExcellentthermalandphotostABIlity
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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