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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A594/100-µl
商品详细StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A594/100-µl
StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A594/100-µl
StressMarq/Anti-PPAR alpha Antibody/SPC-1309D-A594/100-µl
商品编号： SPC-1309D-A594
市场价： ¥6040.00
美元价： 3624.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name PPAR alpha Antibody
Description

Rabbit Anti-Human PPAR alpha Polyclonal

Species Reactivity Human
Applications WB, ICC/IF, ELISA
Antibody Dilution WB (1:1000), ICC/IF (1:500), ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized unphosphorylated peptide derived from human PPAR α around the phosphorylation site of serine 21 (L-E-SP-P-L).
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~52kDa, endogenous levels of total PPAR α protein.
Cite This Product StressMarq Biosciences Cat# SPC-1309, RRID: AB_2713349
Certificate of Analysis A 1:1000 dilution of SPC-1309 was sufficient for detection of PPAR alpha in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names hPPAR Antibody, MGC2237 Antibody, MGC2452 Antibody, NR1C1 Antibody, Nuclear receptor subfamily 1 group C member 1 Antibody, OTTHUMP00000197740 Antibody, OTTHUMP00000197741 Antibody, Peroxisome proliferative activated receptor alpha Antibody, Peroxisome proliferator activated receptor alpha Antibody, Peroxisome proliferator-activated receptor alpha Antibody, PPAR Antibody, PPAR-alpha Antibody, ppara Antibody, PPARA_HUMAN Antibody, PPARalpha Antibody
Cellular Localization Nucleus
Accession Number NP_005027.2
Gene ID 5465
Swiss Prot Q07869
Scientific Background Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPARG is mainly present in adipose tissue, colon and macrophages. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine)
References 1. Chandra, V. et al. (2008) Nature 456 (7220): 350–356.
2. Greene ME et al. (1995) Gene Expr. 4 (4–5): 281–99.
3. Elbrecht A. et al. (1996) Biochem. Biophys. Res. Commun. 224 (2): 431–7.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~52kDa PPAR alpha protein using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:1000. Predicted/Observed Size: ~52kDa.</p>

Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~52kDa PPAR alpha protein using Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PPAR alpha Polyclonal Antibody (SPC-1309) at 1:1000. Predicted/Observed Size: ~52kDa.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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