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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-PP5 Antibody [2E11]/SMC-243D-A594/100-µg
商品详细StressMarq/Anti-PP5 Antibody [2E11]/SMC-243D-A594/100-µg
StressMarq/Anti-PP5 Antibody [2E11]/SMC-243D-A594/100-µg
StressMarq/Anti-PP5 Antibody [2E11]/SMC-243D-A594/100-µg
商品编号: SMC-243D-A594
市场价: ¥6840.00
美元价: 4104.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name PP5 Antibody
Description

Mouse Anti-Human PP5 Monoclonal IgG2a

Species Reactivity Human
Applications WB, ICC/IF
Antibody Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Full length human PP5 protein
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 2E11
Isotype IgG2a
Specificity Detects ~58kDa.
Cite This Product StressMarq Biosciences Cat# SMC-243, RRID: AB_2699592
Certificate of Analysis 1 µg/ml of SMC-243 was sufficient for detection of PP5 in 15 µg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names PPP5 Antibody, PPT Antibody, Protein phosphatase T Antibody, Serine/threonine protein phosphatase 5 Antibody
Research Areas Cell Signaling, Epigenetics, Epigenetics and Nuclear Signaling
Cellular Localization Cytoplasm, Nucleus
Accession Number NP_006238.1
Gene ID 5536
Swiss Prot P53041
Scientific Background Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phosphorylase and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).
References 1. Butzow R., et al. (1988) J Lab Clin Med. 111(2): 249-256.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green)  at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.

<p>Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Lane 1: MW Ladder. Lane 2: Human A431 (15 µg). Lane 3: Human HEK293 (15 µg). Lane 4: Human Jurkat (15 µg). Load: 15 µg protein. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.</p>

Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Lane 1: MW Ladder. Lane 2: Human A431 (15 µg). Lane 3: Human HEK293 (15 µg). Lane 4: Human Jurkat (15 µg). Load: 15 µg protein. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (SMC-243). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (SMC-243) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。