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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-PKC theta Antibody (pSer695)/SPC-1060D-A390/100-µl
商品详细StressMarq/Anti-PKC theta Antibody (pSer695)/SPC-1060D-A390/100-µl
StressMarq/Anti-PKC theta Antibody (pSer695)/SPC-1060D-A390/100-µl
StressMarq/Anti-PKC theta Antibody (pSer695)/SPC-1060D-A390/100-µl
商品编号: SPC-1060D-A390
市场价: ¥6860.00
美元价: 4116.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNamePKCthetaAntibody(pSer695)
Description

RabbitAnti-HumanPKCtheta(pSer695)Polyclonal

SpeciesReactivityHuman
ApplicationsWB,AM
AntibodyDilutionWB(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenAphospho-specificpeptidecorrespondingtoresiduessurroundingSer695ofhumanPKCtheta(AA692-698)
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationPeptideAffinityPurified
ClonalityPolyclonal
SpecificityDetects81.865kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-1060,RRID:AB_2709479
CertificateofAnalysisA1:250dilutionofSPC-1060wassufficientfordetectionofPKCtheta(pSer695)in10µgofJurkatcelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesPKCqAntibody,PRKCQAntibody,PRKCTAntibody,ProteinkinaseCthetaAntibody,KPCT_HUMANAntibody,MGC126514Antibody,MGC141919Antibody,nPKCthetaAntibody,nPKC-thetaAntibody,nPKCthetaAntibody,OTTHUMP00000043364Antibody,OTTHUMP00000043365Antibody,PKC0Antibody,PKC0Antibody,ProteinkinaseCthetatypeAntibody,ProteinKinaseCthetaAntibody
CellularLocalizationCytoplasm,Cellmembrane
AccessionNumberNP_006248
GeneID5588
SwissProtQ04759
ScientificBackgroundCalcium-independent,phospholipid-anddiacylglycerol(DAG)-dependentserine/threonine-proteinkinasethatmediatesnon-redundantfunctionsinT-cellreceptor(TCR)signaling,includingT-cellsactivation,proliferation,differentiationandsurvival,bymediatingactivationofmultipletranscriptionfactorssuchasNF-kappa-B,JUN,NFATC1andNFATC2.InTCR-CD3/CD28-co-stimulatedT-cells,isrequiredfortheactivationofNF-kappa-BandJUN,whichinturnareessentialforIL2production,andparticipatestothecalcium-dependentNFATC1andNFATC2transactivation.MediatestheactivationofthecanonicalNF-kappa-Bpathway(NFKB1)bydirectphosphorylationofCARD11onseveralserineresidues,inducingCARD11associationwithlipidraftsandrecruitmentoftheBCL10-MALT1complex,whichthenactivatesIKKcomplex,resultinginnucleartranslocationandactivationofNFKB1.Mayalsoplayanindirectroleinactivationofthenon-canonicalNF-kappa-B(NFKB2)pathway.InthesignalingpathwayleADIngtoJUNactivation,actsbyphosphorylatingthemediatorSTK39/SPAKandmaynotactthroughMAPkinasessignaling.PlaysacriticalroleinTCR/CD28-inducedNFATC1andNFATC2transactivationbyparticipatingintheregulationofreducedinositol1,4,5-trisphosphategenerationandintracellularcalciummobilization.AftercostimulationofT-cellsthroughCD28canphosphorylateCBLBandisrequiredfortheubiquitinationandsubsequentdegradationofCBLB,whichisaprerequisitefortheactivationofTCR.DuringT-cellsdifferentiation,playsanimportantroleinthedevelopmentofT-helper2(Th2)cellsfollowingimmuneandinflammatoryresponses,and,inthedevelopmentofinflammatoryautoimmunediseases,isnecessaryfortheactivationofIL17-producingTh17cells.MayplayaminorroleinTh1response.UponTCRstimulation,mediatesT-cellprotectivesurvivalsignalbyphosphorylatingBAD,thusprotectingT-cellsfromBAD-inducedapoptosis,andbyup-regulatingBCL-X(L)/BCL2L1levelsthroughNF-kappa-BandJUNpathways.Inplatelets,regulatessignaltransductiondownstreamoftheITGA2B,CD36/GP4,F2R/PAR1andF2RL3/PAR4receptors,playingapositiverolein"outside-in"signalingandgranulesecretionsignaltransduction.MayrelaysignalsfromtheactivatedITGA2BreceptorbyregulatingtheuncouplingofWASPandWIPF1,therebypermittingtheregulationofactinfilamentnucleationandbranchingactivityoftheArp2/3complex.MaymediateinhibitoryeffectsoffreefattyacidsoninsulinsignalingbyphosphorylatingIRS1,whichinturnblocksIRS1tyrosinephosphorylationanddownstreamactivationofthePI3K/AKTpathway.PhosphorylatesMSN(moesin)inthepresenceofphosphatidylglycerolorphosphatidylinositol.PhosphorylatesPDPK1at"Ser-504"and"Ser-532"andnegativelyregulatesitsABIlitytophosphorylatePKB/AKT1.

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 ATTO390
Overview:
  • Highfluorescenceyield
  • LargeStokes-shift(89nm)
  • Goodphotostability
  • Moderatelyhydrophilic
  • Goodsolubilityinpolarsolvents
  • Coumarinderivate,uncharged
  • Lowmolarmass:343.42g/mol 

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission SpectraOpticalProperties:

λex =390nm

λem=479nm

εmax=2.4×104

Φf= 0.90

τfl =5.0ns

Brightness= 21.6

Laser =365or405nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。