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当前位置: 首页 > 产品中心 > Other_buffers > StressMarq/Anti-PCNA Antibody/SPC-1307D-HRP/100-µl
商品详细StressMarq/Anti-PCNA Antibody/SPC-1307D-HRP/100-µl
StressMarq/Anti-PCNA Antibody/SPC-1307D-HRP/100-µl
StressMarq/Anti-PCNA Antibody/SPC-1307D-HRP/100-µl
商品编号: SPC-1307D-HRP
市场价: ¥5800.00
美元价: 3480.00
产地: 美国(厂家直采)
公司:
产品分类: 其它缓冲液
公司分类: Other_buffers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNamePCNAAntibody
Description

RabbitAnti-HumanPCNAPolyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ELISA(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumanPCNA.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~29kDa,endogenouslevelsoftotalPCNAprotein.
CiteThisProductStressMarqBiosciencesCat#SPC-1307,RRID:AB_2713313
CertificateofAnalysisA1:1000dilutionofSPC-1307wassufficientfordetectionofPCNAin10µgofHepG2celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesATLD2Antibody,cb16Antibody,CyclinAntibody,DNApolymerasedeltaauxiliaryproteinAntibody,etID36690.10Antibody,fa28e03Antibody,fb36g03Antibody,HGCN8729Antibody,MGC8367Antibody,Mutagen-sensitive209proteinAntibody,OTTHUMP00000030189Antibody,OTTHUMP00000030190Antibody,PCNAAntibody,Pcna/cyclinAntibody,PCNA_HUMANAntibody,PCNARAntibody,PolymerasedeltaaccessoryproteinAntibody,ProliferatingcellnuclearantigenAntibody,wu:fa28e03Antibody,wu:fb36g03Antibodya
CellularLocalizationNucleus
AccessionNumberNP_872590.1
GeneID5111
SwissProtP12004
ScientificBackgroundProliferatingcellnuclearantigen(PCNA)isaDNAclampthatactsasaprocessivityfactorforDNApolymeraseδineukaryoticcellsandisessentialforreplication.Itisa36kDamoleculethatishighlyconservedbetweenspecies.ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleADIngstrand.
References1.MoldovanGL.etal.(2007)Cell129(4):665–79.
2.WarbrickE.etal.(1998)BioEssays20(3):195–9.
3.GilljamKM.etal.(2009)TheJournalofCellBiology186(5):645–54.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-PCNAPolyclonalAntibody(SPC-1307).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-PCNAPolyclonalAntibody(SPC-1307)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HepG2 cell lysates showing detection of ~29kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:1000. Predicted/Observed Size: ~29kDa.</p>

WesternblotanalysisofHumanHepG2celllysatesshowingdetectionof~29kDaPCNAproteinusingRabbitAnti-PCNAPolyclonalAntibody(SPC-1307).Lane1:HumanHepG2cells.Lane2:HumanHepG2cellstreatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-PCNAPolyclonalAntibody(SPC-1307)at1:1000.Predicted/ObservedSize:~29kDa.

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HRP(Horseradishperoxidase)

Properties:

  • Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
  • Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
  • Catalyzestheconversionof:
    • Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
    • Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
    • Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
  • Highturnoverrateenablesrapidgenerationofastrongsignal
  • 44kDaglycoprotein
  • Extinctioncoefficient:100(403nm)
  • Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。