STRESTMARQ/抗p38抗体/SPC-172D-A565/100-µl-免疫在线蚂蚁淘旗下平台

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STRESTMARQ/抗p38抗体/SPC-172D-A565/100-µl

商品编号： SPC-172D-A565

品牌： StressMarq Biosciences

市场价： ¥5040.00

美元价： 3024.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName p38Antibody

Description

RabbitAnti-Humanp38Polyclonal

SpeciesReactivity Dog,Human,Monkey,Mouse,Rat,Bovine,Chicken,GuineaPig(Caviaporcellus),Hamster,Pig,Rabbit,Sheep

Applications WB,IHC,ICC/IF,IP

AntibodyDilution WB(1:1000),ICC/IF(1:100),IP(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen A20residuesyntheticpeptidebasedonthehumanp38withthecysteineresidueaddedandcoupledtoKLH

Concentration 1mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects~43kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-172,RRID:AB_1055444
CertificateofAnalysis	A1:1000dilutionofSPC-172wassufficientfordetectionofp38in20µgofHeLacelllysatebyECLimmunoblotanalysis.

BIOLOGicalDescription

AlternativeNames	CSAIDBindingprotein1Antibody,CSBP1Antibody,CSBP2Antibody,EXIPAntibody,MAPkinaseMXI2Antibody,MAPkinasep38alphaAntibody,MAPK14Antibody,p38ALPHAAntibody,p38MAPkinaseAntibody,p38mitogenactivatedproteinkinaseAntibody,RKAntibody,SAPK2AAntibody,Stressactivatedproteinkinase2AAntibody
ResearchAreas	Cancer,CellSignaling,Phosphorylation,Post-translationalModifications
CellularLocalization	Cytoplasm,Nucleus
AccessionNumber	NP_001306.1
GeneID	1432
SwissProt	Q16539
ScientificBackground	TheMAPK(mitogenactivatedproteinkinase)comprisesafamilyofubiquitouspraline-directed,protein-serine/threoninekinaseswhichsignaltransductionpathwaysthatcontrolintracellulareventsincludingacuteresponsestohormonesandmajordevelopmentalchangesinorganisms(1).Thissuperfamilyconsistsofstressactivatedproteinkinases(SAPKs);extracellularsignal-regulatedkinases(ERKs);andp38kinases,eachofwhichformsaseparatepathway(2).Thekinasemembersthatpopulateeachpathwayaresequentiallyactivatedbyphosphorylation.Uponactivation,p38MAPK/SAPK2αtranslocatesintothenucleuswhereitphosphorylatesoneormorenuclearsubstrates,effectingtranscriptionalchangesandothercellularprocessesinvolvedincellgrowth,division,differentiation,inflammation,anddeath(3).Specificallyp38alwaysactsasapro-apoptoticfactorwithitsactivationleadingtothereleaseofcytochromecfrommitochondriaandcleavageofcaspase3anditsdownstreameffector,PARP(4).p38MAPKisactivatedbyavarietyofchemicalstressinducersincludinghydrogenperoxide,heavymetals,anisomycin,sodiumsalicylate,LPS,andbiologicalstresssignalssuchastumornecrosisfactor,interleukin-1,ionizingandUVirradiation,hyperosmoticstressandchemotherapeuticdrugs(5).Asaresult,p38alphahasbeenwidelyvalidatedasatargetforinflammatorydiseaseincludingrheumatoidarthritis,COPDandpsoriasis(6)andhasalsobeenimplicatedincancer,CNSanddiabetes(7).
References	1.PearsonG.,etal(2001)EndocrineReviews22(2):153-183. 2.FanY.,etal(2007)Mol.Cells23(1):30-38. 3.HanJ.,etal.(1994)Science265:808-811. 4.VanL.A.,etal.(2004)FasebJ.18:1946−1948. 5.Dengetal.(2003)Cell115:61-70. 6.SalojinK.V.,etal.(2006)JImmunol.176(3):1899-907. 7.MedicherlaS.,etal.(2006)JPharmacolExpTher.318(1):99-107.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-p38PolyclonalAntibody(SPC-172).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-p38PolyclonalAntibody(SPC-172)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Mitochondrion.Cytoplasm.Nucleus.Magnification:100x.(A)DAPI(blue)nuclearstain.(B)Anti-p38Antibody.(C)Composite.

<p>Western blot analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

WesternblotanalysisofHumancancercelllinesshowingdetectionofp38proteinusingRabbitAnti-p38PolyclonalAntibody(SPC-172).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:RabbitAnti-p38PolyclonalAntibody(SPC-172)at1:4000for2hoursatRT.SecondaryAntibody:DonkeyAnti-RabbitIgG:HRPfor1houratRT.

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-p38PolyclonalAntibody(SPC-172).Tissue:HeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-p38PolyclonalAntibody(SPC-172)at1:100for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Mitochondrion.Cytoplasm.Nucleus.Magnification:20x.(A)DAPI(blue)nuclearstain.(B)Anti-p38Antibody.(C)Composite.

ProductCitations(1)

WesternBlot

Atf6αdeficiencysuppressesmicroglialactivationandamelioratespathologyofexperimentalautoimmuneencephalomyelitis.

Ta,H.M.etal.(2016)JNeuRochem.139(6):1124-1137.

PubMedID:27333444ReactivityMouseApplications:WesternBlot

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

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StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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