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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-p27 Kip1 Antibody/SPC-1282D-FITC/100-µl
商品详细StressMarq/Anti-p27 Kip1 Antibody/SPC-1282D-FITC/100-µl
StressMarq/Anti-p27 Kip1 Antibody/SPC-1282D-FITC/100-µl
StressMarq/Anti-p27 Kip1 Antibody/SPC-1282D-FITC/100-µl
商品编号: SPC-1282D-FITC
市场价: ¥5880.00
美元价: 3528.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNamep27Kip1Antibody
Description

RabbitAnti-Humanp27Kip1Polyclonal

SpeciesReactivityHuman
ApplicationsIHC,ELISA
AntibodyDilutionIHC(1:100),ELISA(1:40000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromInternalofhumanp27Kip1.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetectsendogenouslevelsoftotalp27Kip1protein.
CiteThisProductStressMarqBiosciencesCat#SPC-1282,RRID:AB_2712881
CertificateofAnalysisA1:100dilutionofSPC-1282wassufficientfordetectionofp27Kip1inhumanbraintissueusingGoatAnti-RabbitIgGasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesCdki1bAntibody,CDKN1BAntibody,CDKN4Antibody,CyclinDependentKinaseInhibitor1BAntibody,Cyclindependentkinaseinhibitorp27Antibody,Cyclin-dependentkinaseinhibitorp27Kip1Antibody,KIP1Antibody,MEN1BAntibody,MEN4Antibody,p27Antibody,p27Kip1Antibody
ResearchAreasCancer,CellCycle,Cip/Kip
CellularLocalizationCytoplasm,Endosome,Nucleus
AccessionNumberNP_004055.1
GeneID1027
SwissProtP46527
ScientificBackgroundThecyclin-dependentkinaseinhibitor,p27Kip1isatumorsuppressorproteinthatactsinthenucleustoenforcecellcyclecheckpoints.Likeitsrelatives,p57Kip2andp21Waf1/Cip1,theABIlitytoenforcetheG1restrictionpointisderivedfromitsinhibitorybindingtoCDK2/cyclinEandotherCDK/cyclincomplexes.Expressionlevelsofp27areupregulatedinquiescentcellsandincellstreatedwithcAMPorothernegativecellcycleregulators.Downregulationofp27canbeinducedbytreatmentwithinterleukin-2orothermitogens;thisinvolvesphosphorylationofp27anditsdegradationbytheubiquitin-proteasomepathway(1-4).
References1.Lloyd,R.V.etal.(1999)AmJPathol154,313-23.
2.Polyak,K.etal.(1994)GenesDev8,9-22.
3.Kato,J.Y.etal.(1994)Cell79,487-96.
4.Vlach,J.etal.(1997)EMBOJ16,5334-44.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282). Tissue: Brain tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-p27Kip1PolyclonalAntibody(SPC-1282).Tissue:Braintissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-p27Kip1PolyclonalAntibody(SPC-1282)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

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 FITC(Fluorescein)
Overview:
  • Excellentfluorescencequantumyield
  • Highrateofphotobleaching
  • Goodsolubilityinwater
  • Broademissionspectrum
  • pHdependentspectra
  • Molecularformula:C20H12O5
  • Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =494 nm

λem=520 nm

εmax=7.3×104

Φf= 0.92

τfl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。