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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-OCT4 Antibody/SPC-1304D-HRP/100-µl
商品详细StressMarq/Anti-OCT4 Antibody/SPC-1304D-HRP/100-µl
StressMarq/Anti-OCT4 Antibody/SPC-1304D-HRP/100-µl
StressMarq/Anti-OCT4 Antibody/SPC-1304D-HRP/100-µl
商品编号： SPC-1304D-HRP
市场价： ¥5800.00
美元价： 3480.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name OCT4 Antibody
Description

Rabbit Anti-Human OCT4 Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ELISA
Antibody Dilution WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from human OCT3 antibody.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~39kDa, endogenous levels of total OCT3 protein.
Cite This Product StressMarq Biosciences Cat# SPC-1304, RRID: AB_2713259
Certificate of Analysis A 1:1000 dilution of SPC-1304 was sufficient for detection of OCT4 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Octamer binding transcription factor 4 Antibody, MGC22487 Antibody, Oct 3 Antibody, Oct 4 Antibody, Oct-3 Antibody, Oct-4 Antibody, OCT3 Antibody, Oct4 Antibody, Octamer binding protein 3 Antibody, Octamer binding protein 4 Antibody, Octamer binding transcription factor 3 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, Octamer-binding transcription factor 3 Antibody, OTF 3 Antibody, OTF 4 Antibody, OTF-3 Antibody, OTF3 Antibody, OTF4 Antibody, PO5F1_HUMAN Antibody, POU class 5 homeobox 1 Antibody, POU domain class 5 transcription factor 1 Antibody, POU domain transcription factor OCT4 Antibody, POU domain, class 5, transcription factor 1 Antibody, POU-type homeodomain-containing DNA-binding protein Antibody, POU5F1 Antibody
Cellular Localization Cytoplasm, Nucleus
Accession Number NP_002692.2
Gene ID 5460
Swiss Prot Q01860
Scientific Background Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a protein that in humans is encoded by the POU5F1 gene. It is a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types.
References 1. Takeda J. et al. (1992) Nucleic Acids Research 20 (17): 4613–20.
2. Niwa H. et al. (2000) Nature Genetics 24 (4): 372–6.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~34kDa OCT4 protein using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:1000. Predicted/Observed Size: ~34kDa.</p>

Western blot analysis of Human HeLa cell lysates showing detection of ~34kDa OCT4 protein using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:1000. Predicted/Observed Size: ~34kDa.

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HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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