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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-OCT4 Antibody/SPC-1304D-FITC/100-µl
商品详细StressMarq/Anti-OCT4 Antibody/SPC-1304D-FITC/100-µl
StressMarq/Anti-OCT4 Antibody/SPC-1304D-FITC/100-µl
StressMarq/Anti-OCT4 Antibody/SPC-1304D-FITC/100-µl
商品编号: SPC-1304D-FITC
市场价: ¥5880.00
美元价: 3528.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameOCT4Antibody
Description

RabbitAnti-HumanOCT4Polyclonal

SpeciesReactivityHuman,Mouse,Rat
ApplicationsWB,IHC,ELISA
AntibodyDilutionWB(1:1000),IHC(1:100),ELISA(1:10000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenSynthesizedpeptidederivedfromhumanOCT3antibody.
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationAffinityPurified
ClonalityPolyclonal
IsotypeIgG
SpecificityDetects~39kDa,endogenouslevelsoftotalOCT3protein.
CiteThisProductStressMarqBiosciencesCat#SPC-1304,RRID:AB_2713259
CertificateofAnalysisA1:1000dilutionofSPC-1304wassufficientfordetectionofOCT4in10µgofHeLacelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesOctamerbindingtranscriptionfactor4Antibody,MGC22487Antibody,Oct3Antibody,Oct4Antibody,Oct-3Antibody,Oct-4Antibody,OCT3Antibody,Oct4Antibody,Octamerbindingprotein3Antibody,Octamerbindingprotein4Antibody,Octamerbindingtranscriptionfactor3Antibody,Octamer-bindingprotein3Antibody,Octamer-bindingprotein4Antibody,Octamer-bindingtranscriptionfactor3Antibody,OTF3Antibody,OTF4Antibody,OTF-3Antibody,OTF3Antibody,OTF4Antibody,PO5F1_HUMANAntibody,POUclass5homeobox1Antibody,POUdomainclass5transcriptionfactor1Antibody,POUdomaintranscriptionfactorOCT4Antibody,POUdomain,class5,transcriptionfactor1Antibody,POU-typehomeodomain-containingDNA-bindingproteinAntibody,POU5F1Antibody
CellularLocalizationCytoplasm,Nucleus
AccessionNumberNP_002692.2
GeneID5460
SwissProtQ01860
ScientificBackgroundOct-4(octamer-bindingtranscriptionfactor4)alsoknownasPOU5F1(POUdomain,class5,transcriptionfactor1)isaproteinthatinhumansisencodedbythePOU5F1gene.ItisamemberofthePOUfamilyoftranscriptionfactors,hasbeendemonstratedtobevitalfortheformationofself-renewingpluripotentstemcells.DuringembryogenesisexpressionofOct-4islimitedtopluripotentcellsoftheinnercellmass(ICM)thatcontributetotheformationofallfetalcelltypes.
References1.TakedaJ.etal.(1992)NucleicAcidsResearch20(17):4613–20.
2.NiwaH.etal.(2000)NatureGenetics24(4):372–6.

ProductImages

<p>Immunohistochemistry analysis using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:100. The image on the right is treated with the synthesized peptide.</p>

ImmunohistochemistryanalysisusingRabbitAnti-OCT4PolyclonalAntibody(SPC-1304).Tissue:LungCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-OCT4PolyclonalAntibody(SPC-1304)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.

<p>Western blot analysis of Human HeLa cell lysates showing detection of ~34kDa OCT4 protein using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:1000. Predicted/Observed Size: ~34kDa.</p>

WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~34kDaOCT4proteinusingRabbitAnti-OCT4PolyclonalAntibody(SPC-1304).Lane1:HumanHeLacells.Lane2:HumanHeLacellstreatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-OCT4PolyclonalAntibody(SPC-1304)at1:1000.Predicted/ObservedSize:~34kDa.

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 FITC(Fluorescein)
Overview:
  • Excellentfluorescencequantumyield
  • Highrateofphotobleaching
  • Goodsolubilityinwater
  • Broademissionspectrum
  • pHdependentspectra
  • Molecularformula:C20H12O5
  • Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission SpectraOpticalProperties:

λex =494 nm

λem=520 nm

εmax=7.3×104

Φf= 0.92

τfl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。