应力MARQ/NpFR1/SIH-181-1MG/1-mg-免疫在线蚂蚁淘旗下平台

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应力MARQ/NpFR1/SIH-181-1MG/1-mg

商品编号： SIH-181-1MG

品牌： StressMarq Biosciences

市场价： ¥8500.00

美元价： 5100.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName	NpFR1
Description	ReversIBLefluorescenceintensity-basedredoxsensor
MolecularFormula	C₂₃H₂₁N₅O₄
MolecularWeight	431.45

Properties

StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
ProductType	RedoxProbe
Solubility	SolubleinDMSO
Source	Synthetic
Appearance	RedSolid
SMILES	C1(N(C(C2=CC5=C(C3=CC=CC1=C23)N(C4=NC(NC(C4=N5)=O)=O)CCC)=O)CCCC)=O
InChI	InChI=1S/C23H21N5O4/c1-3-5-10-28-21(30)13-8-6-7-12-16(13)14(22(28)31)11-15-18(12)27(9-4-2)19-17(24-15)20(29)26-23(3
InChIKey	C5(C2=C1C(=CC4=C(C1=CC=C2)N(C3=NC(NC(C3=N4)=O)=O)CCC)C(N5CCCC)=O)=O
SafetyPhrases	Classification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProduct	NpFR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-181)

BIOLOGicalDescription

ResearchAreas	Cancer,OxidativeStress
ScientificBackground	NpFR1,ornapthalimide-flavinredoxsensor1,isanovelflavinmoleculethatexhibitsagreaterthan100-foldincreaseinfluorescenceuponoxidation,andiseasilyreversible.Intheoxidativeform,isshowsamaximumabsorptionat395and463nm,maxadmissionat545nm.Treatmentwithamildreducingagent(includingsodiumthiosulfate,sodiumcyanoborohydride,DTTandglutathione)gavethereducedformwhichexhibited110-foldlowerabsorbance,and125-foldloweremission.Re-oxidationtoitsoriginalfluorescencecanbeachievedbyairorbyhydrogenperoxide.
References	1.YeowJ.,KaurA.K.,AnscombM.D.,andNewE.J.(2014)Chem.Commun.50:8181-8184.

ProductImages

<p>Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 µM. Excitation: 405 or 488 nm (not shown). Emission: 490 – 600 nm, with peak at 545 nm.</p>

FluorescencebehaviorofNpFR1(SIH-181)intheoxidized(black)andreduced(red)forms,using50µM.Excitation:405or488nm(notshown).Emission:490–600nm,withpeakat545nm.

<p>Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ChemicalstructureofNpFR1(SIH-181),areversiblefluorescenceintensity-basedredoxsensor.ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceemissionofNpFR1(SIH-181,5mM,lex=405nm)withtheincrementaladditionofsodiumdithionite.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceresponseofNpFR1(SIH-181)tocyclesofoxidationandreduction.Reductionwasachievedwithsodiumdithionite(100mM)followedbyre-oxidationwith250mMH2O2.Spectrawererecorded5minaftertheadditionofreducingandoxidisingagents.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ImagingofNpFR1(SIH-181)in3T3-L1preADIpocytestreatedwith(a)vehiclecontrol(b)H2O2(100mM,2min),(c)NpFR1(50mM,2h)and(d)NpFR1(50mM,2h)followedbyH2O2(100mM,2min).Scalebarrepresents50mm,lex=405nm.(e)Integratedemissionfrom510nmto610nm.Valuesarethemeanratiogeneratedfromtheintensityfromfivefieldsofcells.Errorbarsrepresentstandarderrormeasurement(s.e.m.).ThelayoutofthisimagewasmodifiedforoptimaldisplayfromtheoriginalChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

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品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

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