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StressMarq/MOLECULAR SIGNATURE®硝基酪氨酸ELISA试剂盒/SKT-126-96/96孔
StressMarq/MOLECULAR SIGNATURE®硝基酪氨酸ELISA试剂盒/SKT-126-96/96孔
商品编号：
SKT-126-96
市场价：
¥7820.00
美元价：
4692.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | NitrotyrosineELISAKit |
| Description | ColorimetricdetectionofNitrotyrosine |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,Plasma,Serum,Urine |
| DetectionMethod | ColorimetricAssay |
| AssayType | CompetitiveELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateNitrotyrosineinsamples. |
| Sensitivity | 50nM |
| AssayRange | 62.5-8000nM |
| Precision | Intra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%. |
| IncubationTime | 1hour |
| NumberofSamples | 38samplesinduplicate |
| OtherResources | KitBooklet,MSDS,CalculationsWorksheet |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||
| AssayOverview | 1.PreparestandardandsamplesintheSampleandStandardDiluent.2.Add50µLofpreparedstandardsandsamplesintriplicatetoappropriatewells.3.Add50µLofthedilutedantibodypreparationtotheappropriatewells.4.CoverplatewithPlateCoverandincubateatroomtemperature(20-25°C)for1hour.5.Washplate4timeswith1XWashBuffer.6.Add100µLofTMBSubstratetoeachwell.7.Coverplateanddeveloptheplateinthedarkatroomtemperaturefor30minutes.8.Add100µLofStopSolutiontoeachwell.9.Measureabsorbanceonaplatereaderat450nm.10.Plotthestandardcurveandcalculatesampleconcentrations. | ||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | NitrotyrosineELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-126) |
BIOLOGicalDescription
| AlternativeNames | NitrotyrosineELISAKit,3-NitrotyrosineELISAKit,3-Nitro-L-tyrosineELISAKit,3NitrotyrosineELISAKit,3-NTELISAKit,3NTELISAKit |
| ResearchAreas | Cancer,Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience,Nitration,OxidativeStress,Parkinson"sDisease,Post-translationalModifications |
| ScientificBackground | NitrotyrosinehasbeenidentifiedasaMarkerofinflammationandNOproduction.NitrotyrosineisformedinpresenceoftheactivemetaboliteNO.Variouspathwaysincludingtheformationofperoxinitriteleadtonitrotyrosineproduction.Sincenitrotyrosineisastableendproductofperoxynitriteoxidation,assessmentofitsplasmaconcentrationmaybeusefulasamarkerofNO-dependentdamageinvivo.SinceNOXisonlyanindicatorforenhancedNOproduction,proteinassociatednitrotyrosinemightbeamoresuitablemarkerfordamageinducedbyreactivenitrogenintermediatesderivedfromNO.FurThermore,mostproteinshavealongerhalflifeinthecirculationthanNOXlevels.Thepresenceofnitrotyrosinehasbeendetectedinvariousinflammatoryprocessesincludingatheroscleroticplaques,celiacdisease,rheumatoidarthritis,chronicrenalfailureandsepticshock.Innormalplasmalow,undetectable,levelsofnitrotyrosinearepresent.Nitrosylationoftheaminoacidtyrosineoccursbothforfreetyrosineandforproteinboundtyrosine. |
ProductImages
TypicalStandardCurvefortheNitrotyrosineELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-126.AssayType:CompetitiveELISA.DetectionMethod:ColorimetricAssay.AssayRange:62.5–8000nM.
DiagramoftheCompetitiveELISA
DiagramofthePreparationofNitrotyrosineStandards
DiagramoftheTriplicateSamplePlateFormat
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
