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STRESS MARQ/STRESS XPRESS®一氧化氮检测试剂盒/SKT-212-96/2-x-96-well
STRESS MARQ/STRESS XPRESS®一氧化氮检测试剂盒/SKT-212-96/2-x-96-well
商品编号：
SKT-212-96
市场价：
¥5480.00
美元价：
3288.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | NitricOxideDetectionKit |
| Description | Colorimetricdetectionofnitrateandnitrite |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Buffer,Celllysates,Plasma,Saliva,Serum,TissueCultureMedia,Urine,Water |
| DetectionMethod | ColorimetricAssay |
| AssayType | IndirectQuantitativeAssay |
| Utility | ColorimetricassayusedtoquantitativelymeasureNitrateandNitritepresentinavarietyofsamples. |
| Sensitivity | 2.63µMintheNitriteand1.02µMintheTotalNitricOxide |
| AssayRange | 3.125-200µM |
| Precision | IntraAssayPrecision:ThreesampleswerefurtherdilutedinAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedNitriteorTotalNOconcentrationswere:Sample1(NitriteConcentration)-45.1µM,4.4%CV,Sample1(TotalNOConcentration)-70.5µM,6.8%CVSample2(NitriteConcentration)-73.3µM,9.1%CV,Sample1(TotalNOConcentration)-107.4µM,4.4%CVSample3(NitriteConcentration)-132.7µM,1.3%CV,Sample1(TotalNOConcentration)-157.8µM,1.8%CVInterAssayPrecision:ThreesampleswerefurtherdilutedinAssayBufferandruninduplicatesintwentyassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedNitriteorTotalNOconcentrationswere:Sample1(NitriteConcentration)-44.1µM,3.1%CV,Sample1(TotalNOConcentration)-68.8µM,7.4%CVSample2(NitriteConcentration)-66.4µM,4.0%CV,Sample1(TotalNOConcentration)-112.1µM,5.7%CVSample3(NitriteConcentration)-126.7µM,6.3%CV,Sample1(TotalNOConcentration)-154.48µM,4.1%CV |
| IncubationTime | 30minutes |
| NumberofSamples | 88samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||
| ProductType | DetectionKits | ||||||||||||||||||||||||||||||
| AssayOverview | TheNitricOxideDetectionKitisdesignedtoquantitativelymeasureNitrateandNitritepresentinavarietyofsamples.NitricOxidecontentisderivedfromthesumofNitrate(-NO3)andNitrite(-NO2).BothNitrateandNitritestandardsareprovidedtogeneratestandardcurvesfortheassayandallsamplesshouldbereadofftheappropriatestandardcurve.ForNitritedetection,samplesaremixedwiththeColorReagentsAandBandincubatedatroomtemperaturefor5minutes.Thecoloredproductisreadat550–570nm.TheconcentrationofNitriteinthesampleiscalculated,aftermakingasuitablecorrectionforanydilutionofthesample,usingsoftwareavailablewithmostplatereaders.TotalNitricOxidecontentismeasuredafterthesampleisincubatedwithNitrateReductaseandNADH.ThereductaseincombinationwithNADHreducesNitratetoNitrite.Aftera20minuteincubationatroomtemperature,ColorReagentsAandBareaddedandincubatedatroomtemperaturefor5minutes.ThecoloredproductisreadandcalculatedaswiththeNitritedeterminationabove.TheconcentrationofNitrateinthesampleiscalculatedbysubtractingthemeasuredNitriteconcentrationfromtheTotalNitricOxideconcentrationforthesample. | ||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | NitricOxideDetectionKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-212) |
BIOLOGicalDescription
| AlternativeNames | Nitrogenmonoxide;Nitrogen(II)oxideDetectionKit |
| ResearchAreas | CellSignaling,Neuroscience,Post-translationalModifications |
| ScientificBackground | Nitricoxide(NO)isadiffusIBLe,transient,reactivemoleculethathasphysiologicaleffectsinthepicomolar-to-micromolarrange.Actingthroughsolubleguanylatecyclaseactivation,NOisanimportantphysiologicalregulatorofthecardiovascular,nervous,andimmunologicalsystems(1).NOisbio-availablebytworoutes.ItcanbeendogenouslygeneratedbyconstitutiveorinducedenzymeslikeNitricOxideSynthaseoritcanbeorallyingestedasnitrates/nitritesforrapiduptakeintocirculationandsubsequentconversion(2).Thereactivenatureofnitricoxideallowsittoactasacytotoxicfactorwhenreleasedduringanimmuneresponsebycellssuchasmacrophages.ThereactivityalsoallowsNOtobeeasilyconvertedtoatoxicrADIcalthatcanproducenitrosativedamagetocells,organellesandmoleculessuchasDNA.Nitrosaylationhowevercanbearegulatedpost-translationalmodificationincellsignaling(3).Thebalanceanddynamicsoftheregulatory/damagefacetsofNOaremajorforcesinmitochondrialsignalinganddysfunction(4).NOislinkednotonlytocoronaryheartdisease,endothelialdysfunctions,erectiledysfunction,andneurologicaldisorders,butalsodiabetes,chronicperiodontitis,autism,cancer,andassortedage-relateddiseases(5-9).ThephysicalpropertiesofNitricOxidemakeitchallengingfordirectdetectionmethods.However,colorimetricmethodscanbeappliedtomeasureitsstablebreak-downproductsnitrate(-NO3)andnitrite(-NO2)(10). |
| References | 1.Moncada,SandEAHiggs.(1991)Eur.J.Clin.Invest.,21:361-374. 2.Kapil,V.etal.(2010)Heart,96:1703-1709. 3.Seth,DandStamler,JS.(2007)Curr.Opin.Chem.Biol.,15:1-8. 4.Eursalimsky,JDandMoncada,S.(2007)ATVB27:2524-2531. 5.Knott,ABandBossy-Wetzel,E.(2010)Diab.Obes.Metab.12(Suppl2):126-133. 6.VanDyke,K.etal.(2010)Ann.N.Y.Acad.Sci.1203:138-145. 7.Reher,VSG.etal.(2007)J.OralSci.49(4):271-276. 8.Sogut,S.etal.(2003)Clin.Chim.Acta331:111-117. 9.Balam.E.etal.(2002)Jpn.J.Clin.Oncol.32(5):162-166. 10.Moshage,H.(1997)Clin.Chem.43(4):553-556. |
ProductImages
TypicalStandardCurveforNitricOxideDetectionKitStressXpress®–SKT-212.AssayType:CoupledEnzymeAssay.DetectionMethod:ColorimetricAssay.AssayRange:3.125–200uM.
LinearitywasdeterminedbytakingtwohumanurinesampleswithknownNitriteandTotalNitricOxideConcentrationsandmixingthemingivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
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品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
