
Overview:
Product Name | NFATC1 antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human NFATC1 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide of Human NFATC1 (110-210 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects 90 kDa, band at ~60 kDa is another isoform. |
Cite This Product | StressMarq Biosciences Cat# SPC-727, RRID: AB_2706257 |
Certificate of Analysis | A 1:1000 dilution of SPC-727 was sufficient for detection of NFATC1 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | NFAT2 Antibody, NFATc Antibody, OR56B4 Antibody, Olfactory receptor OR11-67 Antibody, NF-ATc1 Antibody, OR11-67 Antibody, NFAT transcription complex cytosolic component Antibody, NFATc1 Antibody, NF-ATc Antibody, Nuclear factor of activated T-cells cytoplasmic 1 Antibody, NFATC Antibody, NFATC1 Antibody |
Research Areas | Adaptive Immunity, Cardiogenesis, Cardiovascular System, Cell Signaling, Epigenetics and Nuclear Signaling, Heart, Immunology, T Cells |
Cellular Localization | Cytoplasm, Nucleus |
Accession Number | NP_001265598.1 |
Gene ID | 4772 |
Swiss Prot | O95644 |
Product Images

Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.
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HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
HRP Datasheet