StressMarq/Anti-MDM2 Antibody/SPC-1329D-A488/100-µl-免疫在线蚂蚁淘旗下平台

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StressMarq/Anti-MDM2 Antibody/SPC-1329D-A488/100-µl

商品编号： SPC-1329D-A488

品牌： StressMarq Biosciences

市场价： ¥6040.00

美元价： 3624.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

Product Name MDM2 Antibody

Description

Rabbit Anti-Human MDM2 Polyclonal

Species Reactivity Human, Rat

Applications WB, IHC, ICC/IF, ELISA

Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthesized peptide derived from human MDM2.

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects endogenous levels of total MDM2 protein.
Cite This Product	StressMarq Biosciences Cat# SPC-1329, RRID: AB_2713709
Certificate of Analysis	A 1:1000 dilution of SPC-1329 was sufficient for detection of MDM2 in 10 µg of SKOV3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	ACTFS antibody, Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, Hdm2 antibody, HDMX antibody, MDM 2 antibody, MDM2 antibody, MDM2 oncogene E3 ubiquitin protein ligase antibody, Mdm2 p53 E3 ubiquitin protein ligase homolog antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody
Cellular Localization	Cytoplasm, Nucleus
Accession Number	NP_002383.2
Gene ID	4193
Swiss Prot	Q00987
Scientific Background	Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
References	1. Uhrinova S et al. (2005) J. Mol. Biol. 350 (3): 587–98. 2. Oliner JD et al. (1992) Nature 358 (6381): 80–3. 3. Wade M. et al. (2006) J. Biol. Chem. 281 (44): 33036–44. 4. Ebrahim, M. et al. (2015) Histology and histopathology, 30(11), 1271-1282. 30(11):1271-1282.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Western blot analysis of Human SKOV3 cell lysates showing detection of ~90kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Lane 1: Human SKOV3 cells. Lane 2: Human SKOV3 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:1000. Predicted/Observed Size: ~90kDa.</p>

Western blot analysis of Human SKOV3 cell lysates showing detection of ~90kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Lane 1: Human SKOV3 cells. Lane 2: Human SKOV3 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:1000. Predicted/Observed Size: ~90kDa.

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ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

品牌介绍

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。

公司介绍