
Overview:
Product Name | MBP Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human MBP Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Peptide sequence around aa.291~295(G-G-R-D-S | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Affinity Purified |
Clonality | Polyclonal |
Isotype | IgG |
Specificity | Detects ~19kDa and ~26kDa, endogenous level of total MBP(myelin basic protein) protein. |
Cite This Product | StressMarq Biosciences Cat# SPC-1301, RRID: AB_2713205 |
Certificate of Analysis | A 1:1000 dilution of SPC-1301 was sufficient for detection of MBP in 10 µg of Brain cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | GDB Antibody, Golli MBP Antibody, Golli MBP; myelin basic protein Antibody, Hemopoietic MBP Antibody, HMBPR Antibody, HUGO Antibody, MBP Antibody, MBP_HUMAN Antibody, MGC99675 Antibody, MLD Antibody, Myelin A1 protein Antibody, Myelin basic protein Antibody, Myelin Deficient Antibody, Myelin membrane encephalitogenic protein Antibody, OTTHUMP00000163776 Antibody, OTTHUMP00000174387 Antibody, OTTHUMP00000174388 Antibody, SHI Antibody, Shiverer Antibody, SP Antibody |
Cellular Localization | Membrane |
Accession Number | NP_002376.1 |
Gene ID | 4155 |
Swiss Prot | P02686 |
Scientific Background | Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the nervous system. The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. |
References |
1. Sakamoto Y. et al. (1987) J. Biol. Chem. 262 (9): 4208–14. 2. Deber CM and Reynolds SJ. (1991) Clin. Biochem. 24 (2): 113–34. 3. Inouye H and Kirschner DA. (1991) J. Neurosci. Res. 28 (1): 1–17. 4. Eylar EH et al. (1971) J. Biol. Chem. 246 (18): 5770–84. 5. Berger T et al. (2003) N. Engl. J. Med. 349 (2): 139–45. |
Product Images

Western blot analysis of Rat, Mouse Brain cell lysates showing detection of ~14-33kDa MBP protein using Rabbit Anti-MBP Polyclonal Antibody (SPC-1301). Lane 1: Rat Brain. Lane 2: Mouse Brain. Lane 3: Rat Hippocampus. Primary Antibody: Rabbit Anti-MBP Polyclonal Antibody (SPC-1301) at 1:1000. Predicted/Observed Size: ~14-33kDa.
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ATTO 680 | ||
Overview:
ATTO 680 Datasheet | ![]() | Optical Properties: λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |