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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-LRRK2/Dardarin Antibody [S138-6]/SMC-446D-P594/100-µg
商品详细StressMarq/Anti-LRRK2/Dardarin Antibody [S138-6]/SMC-446D-P594/100-µg
StressMarq/Anti-LRRK2/Dardarin Antibody [S138-6]/SMC-446D-P594/100-µg
StressMarq/Anti-LRRK2/Dardarin Antibody [S138-6]/SMC-446D-P594/100-µg
商品编号: SMC-446D-P594
市场价: ¥6960.00
美元价: 4176.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name LRRK2/Dardarin Antibody
Description

Mouse Anti-Human LRRK2/Dardarin Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, ICC/IF
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2. 83% identical in mouse and rat. No significant identity with LRRK1.
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S138-6
Isotype IgG1
Specificity Detects >~200kDa.
Cite This Product StressMarq Biosciences Cat# SMC-446, RRID: AB_2701775
Certificate of Analysis 1 µg/ml of SMC-446 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Leucine-rich repeat kinase 2 Antibody, RIP7 Antibody, PARK8 Antibody, Dardarin Antibody, ROCO 2 Antibody, RIPK7 Antibody, Leucine-rich repeat serine/threonine-protein kinase 2 Antibody, augmented in rheumatoid arthritis 17 Antibody
Research Areas Cell Signaling, Neurodegeneration, Neuroscience, Parkinson's Disease, Phosphorylation, Post-translational Modifications
Cellular Localization Cytoplasm, Axon, Cell projection, Cytoplasmic Vesicle, Dendrite, Endoplasmic Reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Mitochondrion
Accession Number NP_940980
Gene ID 120892
Swiss Prot Q5S007
Scientific Background LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRRK2/Dardarin Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRRK2/Dardarin Antibody (D) Composite.

<p>Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.</p>

Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S138-6 (SMC-446). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-446) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.

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 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission SpectraOptical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。