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当前位置： 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-LC3A Antibody/SPC-216A-A594/50-µg
商品详细StressMarq/Anti-LC3A Antibody/SPC-216A-A594/50-µg
StressMarq/Anti-LC3A Antibody/SPC-216A-A594/50-µg
StressMarq/Anti-LC3A Antibody/SPC-216A-A594/50-µg
商品编号： SPC-216A-A594
市场价： ¥6640.00
美元价： 3984.00
产地： 美国(厂家直采)
公司：
产品分类： 酸碱缓冲液
公司分类： acid_base_buffer_solution
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Overview:

Product Name LC3A Antibody
Description

Rabbit Anti-Human LC3A Polyclonal

Species Reactivity Human, Mouse, Rat
Applications WB
Antibody Dilution WB (1:5000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Peptide derived from N-terminal region of human LC3A protein
Concentration 0.25 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS, 0.09% sodium azide
Storage Temperature 4ºC
Shipping Temperature Blue Ice or 4ºC
Purification Salt precipitated
Clonality Polyclonal
Specificity Specific for LC3A. Does not detect LC3B, LC3C, GABARAP, GABARAPL1 or GABARAPL2. Detects LC3A-I and LC3A-II.
Cite This Product StressMarq Biosciences Cat# SPC-216, RRID: AB_2704397
Certificate of Analysis A 1:5000 dilution of SPC-216 was sufficient for detection of LC3A in lysate from cells expressing recombinant GST-tagged LC3A by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names MAP1LC3A Antibody, ATG8F Antibody, Autophagy related protein LC3 A Antibody, Autophagy related ubiquitin like modifier LC3 A Antibody, MAP1LC3A Antibody, Microtubule associated protein 1 light chain 3 alpha Antibody, LC3 A Antibody, LC3 Antibody, MAP1 light chain 3 like protein 1 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B LC3 A Antibody, MAP1ALC3 Antibody
Research Areas Cancer, Autophagy, Cardiovascular System, Cell Signaling, Heart, Neuroscience
Cellular Localization Cytoplasm, Autophagosome, autophagosome membrane, Cytoplasmic Vesicle, Cytoskeleton, Endomembrane system, Lipid-Anchor
Accession Number NP_852610.1
Gene ID 84557
Swiss Prot Q9H492
Scientific Background Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
References 1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88.
2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.

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 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
公司介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒
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