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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-Kv3.1 Antibody [S16B-8]/SMC-313D-A594/100-µg
商品详细StressMarq/Anti-Kv3.1 Antibody [S16B-8]/SMC-313D-A594/100-µg
StressMarq/Anti-Kv3.1 Antibody [S16B-8]/SMC-313D-A594/100-µg
StressMarq/Anti-Kv3.1 Antibody [S16B-8]/SMC-313D-A594/100-µg
商品编号: SMC-313D-A594
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Kv3.1 Antibody
Description

Mouse Anti-Rat Kv3.1 Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, AM
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Fusion protein amino acids 437-585 (C-terminus) or rat Kv3.1b
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number S16B-8
Isotype IgG1
Specificity Detects ~110kDa. Weakly reactive in human samples.
Cite This Product StressMarq Biosciences Cat# SMC-313, RRID: AB_2131475
Certificate of Analysis 1 µg/ml of SMC-313 was sufficient for detection of Kv3.1b in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Krc2 1 Antibody, NGK2 Antibody, KCNC1 Antibody, voltage gated potassium channel subunit Kv3.1 Antibody, Kv4 Antibody
Research Areas Ion Channels, Neuroscience, Potassium Channels, Voltage-Gated Potassium Channels
Cellular Localization Membrane
Accession Number NP_036988
Gene ID 25327
Swiss Prot P25122
Scientific Background Potassium voltage-gated channel, Shaw-related subfamily, member 1, also known as KCNC1 or Kv3.1, is a human gene. The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily (1). The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Kv3.1b has been extensively tested in the auditory regions of mammals, and the decline of Kv3.1b expression appears to correlate with the functional decline in the medial olivocochlear efferent system (2). Other research shows potential for Kv3.1b channels to be oxygen sensors (3).
References 1. Xu M., et al. (2007) J. Neuroscience. 27(51): 14158-14170.
2. Zettel M.L., Zhu X., O’Neill W.E., Frisina R.D. (2007) J Assoc Res Otolaryngol. 8(2): 280-293.
3. Osipenko O.N., Tate R.J. and Gurney A.M. (2000) Circ Res. 86(5): 534-540.

Product Images

<p>Immunohistochemistry analysis using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.</p>

Immunohistochemistry analysis using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

<p>Western Blot analysis of Rat brain lysates showing detection of Kv3.1 Potassium Channel protein using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.</p>

Western Blot analysis of Rat brain lysates showing detection of Kv3.1 Potassium Channel protein using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Citations (2)

Other Citations

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Human Applications: Antibody Microarray

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity Mouse Applications: Antibody Microarray

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOptical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。