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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > StressMarq/Anti-Ki67 Antibody/SPC-1327D-A488/100-µl
商品详细StressMarq/Anti-Ki67 Antibody/SPC-1327D-A488/100-µl
StressMarq/Anti-Ki67 Antibody/SPC-1327D-A488/100-µl
StressMarq/Anti-Ki67 Antibody/SPC-1327D-A488/100-µl
商品编号: SPC-1327D-A488
市场价: ¥6040.00
美元价: 3624.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Ki67 Antibody
Description

Rabbit Anti-Human Ki67 Polyclonal

Species Reactivity Human
Applications IHC, ICC/IF, ELISA
Antibody Dilution IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthesized peptide derived from C-terminal of human ki67.
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects endogenous levels of total ki67protein.
Cite This Product StressMarq Biosciences Cat# SPC-1327, RRID: AB_2713673
Certificate of Analysis A 1:100 dilution of SPC-1327 was sufficient for detection of Ki67 in human lung carcinoa tissue using Goat Anti-Rabbit IgG as the secondary antibody.

Biological Description

Alternative Names Antigen identified by monoclonal antibody Ki 67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody
Cellular Localization Chromosome, Nucleus
Accession Number NP_002408.3
Gene ID 4288
Swiss Prot P46013
Scientific Background Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.
References 1. Schonk DM. et al. (1989) Hum. Genet. 83 (3): 297–9.
2. Bullwinkel J. et al. (2006) J. Cell. Physiol. 206 (3): 624–35.
3. Rahmanzadeh R. et al. (2007) Cell Prolif. 40 (3): 422–30.
4. Scholzen T and Gerdes J (2000) J. Cell. Physiol. 182 (3): 311–22.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide.

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  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。