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商品详细StressMarq/Anti-IRF3 Antibody/SPC-1298D-STR/100-µl
StressMarq/Anti-IRF3 Antibody/SPC-1298D-STR/100-µl
StressMarq/Anti-IRF3 Antibody/SPC-1298D-STR/100-µl
商品编号: SPC-1298D-STR
市场价: ¥6000.00
美元价: 3600.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name IRF3 Antibody
Description

Rabbit Anti-Human IRF3 Polyclonal

Species Reactivity Human
Applications WB, IHC, ICC/IF
Antibody Dilution WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A synthesized peptide derived from human IRF3
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Isotype IgG
Specificity Detects ~47kDa, endogenous levels of total IRF3
Cite This Product StressMarq Biosciences Cat# SPC-1298, RRID: AB_2713169
Certificate of Analysis A 1:1000 dilution of SPC-1298 was sufficient for detection of IRF3 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Interferon regulatory factor 3 Antibody IRF 3 Antibody IRF-3 Antibody IRF3 Antibody IRF3_HUMAN Antibody MGC94729 Antibody
Cellular Localization Cytoplasm, Nucleus
Accession Number NP_001562.1
Gene ID 3661
Swiss Prot Q14653
Scientific Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. It can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.
References 1. Hiscott J. et al. (1999) J. Interferon Cytokine Res. 19 (1): 1–13.
2. Lin R. et al. (1999) Mol Cell Biol 19 (2): 959–66.
3. Yoneyama M. et al. (2002) J. Interferon Cytokine Res. 22 (1): 73–6.
4. Collins S.E. et al. (2004) J Virol 78 (4): 1706–17.
5. Hou F. et al. (2011) Cell 146 (3): 448–61.

Product Images

<p>Western blot analysis of Human K562 cell lysates showing detection of ~57kDa IRF3 protein using Rabbit Anti-IRF3 Polyclonal Antibody (SPC-1298). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-IRF3 Polyclonal Antibody (SPC-1298) at 1:1000. Predicted/Observed Size: ~57kDa.</p>

Western blot analysis of Human K562 cell lysates showing detection of ~57kDa IRF3 protein using Rabbit Anti-IRF3 Polyclonal Antibody (SPC-1298). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-IRF3 Polyclonal Antibody (SPC-1298) at 1:1000. Predicted/Observed Size: ~57kDa.

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Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。